Genetic Complexity of Cellulose Synthase A Gene Function in Arabidopsis Embryogenesis
| Autor: | Gerhard K. H. Przemeck, Dirk Inzé, Rachel Lau, Nancy Terryn, Thomas Berleth, Tom Beeckman, George Stamatiou, Riet De Rycke |
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| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Cell division Physiology Molecular Sequence Data Arabidopsis Plant Science medicine.disease_cause Plant Roots Gene Expression Regulation Enzymologic Gene Expression Regulation Plant Sequence Homology Nucleic Acid Gene expression Genetics medicine Arabidopsis thaliana Gene family Amino Acid Sequence Gene In Situ Hybridization Mutation Microscopy Confocal Base Sequence Sequence Homology Amino Acid biology Arabidopsis Proteins Gene Expression Regulation Developmental biology.organism_classification Isoenzymes Microscopy Electron Phenotype Glucosyltransferases Seeds Cell Division Plant Shoots Research Article |
| Zdroj: | Plant Physiology. 130:1883-1893 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.102.010603 |
| Popis: | The products of the cellulose synthase A (CESA) gene family are thought to function as isoforms of the cellulose synthase catalytic subunit, but for most CESA genes, the exact role in plant growth is still unknown. Assessing the function of individual CESA genes will require the identification of the null-mutant phenotypes and of the gene expression profiles for each gene. Here, we report that only four of 10 CESA genes,CESA1, CESA2, CESA3, andCESA9 are significantly expressed in the Arabidopsis embryo. We further identified two new mutations in the RADIALLY SWOLLEN1 (RSW1/CESA1) gene of Arabidopsis that obstruct organized growth in both shoot and root and interfere with cell division and cell expansion already in embryogenesis. One mutation is expected to completely abolish the enzymatic activity of RSW1(CESA1) because it eliminated one of three conserved Asp residues, which are considered essential for β-glycosyltransferase activity. In this presumed null mutant, primary cell walls are still being formed, but are thin, highly undulated, and frequently interrupted. From the heart-stage onward, cell elongation in the embryo axis is severely impaired, and cell width is disproportionally increased. In the embryo, CESA1,CESA2, CESA3, and CESA9are expressed in largely overlapping domains and may act cooperatively in higher order complexes. The embryonic phenotype of the presumedrsw1 null mutant indicates that the RSW1(CESA1) product has a critical, nonredundant function, but is nevertheless not strictly required for primary cell wall formation. |
| Databáze: | OpenAIRE |
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