Cytoprotection against lipid hydroperoxides correlates with increased glutathione peroxidase activities, but not selenium uptake from different selenocompounds
| Autor: | Marcel Leist, Regina Brigelius-Flohé, Angelika Elsner, Manfred Schultz, Dieter Gawlik, Stefanie Maurer |
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| Rok vydání: | 1999 |
| Předmět: |
Lipid Peroxides
Carcinoma Hepatocellular Time Factors Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biological Availability chemistry.chemical_element Selenium in biology medicine.disease_cause Biochemistry Antioxidants Inorganic Chemistry Selenium Tumor Cells Cultured medicine Humans Vitamin E Tocopherol chemistry.chemical_classification Glutathione Peroxidase Dose-Response Relationship Drug Glutathione peroxidase Biochemistry (medical) food and beverages General Medicine Cytoprotection Bioavailability chemistry Oxidative stress |
| Zdroj: | Biological Trace Element Research. 68:159-174 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/bf02784404 |
| Popis: | Cells cultivated under standard conditions were highly deficient in tocopherol, selenium, and glutathione peroxidase (GPx) activities. We investigated whether and to what extent the addition of different selenocompounds to growth media would alter biochemical, physiological, and pathophysiological parameters of cultured liver cells. Cellular uptake of selenium, GPx activities, and cytoprotection were measured and compared in human hepatoma cells (HepG2). Selenite and selenocystine were Se donors of high bioavailability (i.e., with these culture supplements, the increased Se uptake, induction of GPx isoenzymes, and protection of treated cells from lipid hydroperoxides were well correlated). In contrast, selenium from selenomethionine was incorporated into cellular proteins but had no effect on GPx activities or cytoprotection. The data show that not all selenium donors provide selenium, which is bioactivated to act as antioxidant. Thus, cellular selenium content, in general, did not correlate with cytoprotective activity of this trace element. However, cellular GPx activities at different times, with different concentrations, and with different Se donors always correlated with protection from lipid hydroperoxides and may, thus, represent a more reliable parameter to define adequate Se supply. |
| Databáze: | OpenAIRE |
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