Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay

Autor: Rodney Colina, Carlos Schild, Federico Giannitti, Viviana Parreño, Matías Victoria, Franklin Riet-Correa, Felipe Castells, Daniel Castells, Rubén D. Caffarena, María Laura Casaux, Matías Castells
Rok vydání: 2020
Předmět:
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Infection, Genetics and Evolution 86 : 104613 (December 2020)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
ISSN: 1567-1348
DOI: 10.1016/j.meegid.2020.104613
Popis: Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay. Instituto de Virología Fil: Castells, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay. Fil: Castells, Matías. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Caffarena, Rubén Darío. Universidad de la República. Facultad de Veterinaria; Uruguay. Fil: Casaux, María Laura. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Castells, Felipe. Profesional independiente; Uruguay Fil: Castells, Daniel. Secretariado Uruguayo de la Lana. Centro de Investigación y Experimentación Dr. Alejandro Gallinal; Uruguay. Fil: Victoria, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay. Fil: Riet‑Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Databáze: OpenAIRE
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