Mucosal Invariant T cells are Diminished in Very Early-Onset Inflammatory Bowel Disease
| Autor: | Ying Dou, Kathleen E. Sullivan, Trusha Patel, Maire A. Conrad, Kelly Maurer, Judith R. Kelsen, Rawan Shraim |
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| Rok vydání: | 2021 |
| Předmět: |
Cell type
T-Lymphocytes Lymphocyte Population Disease Inflammatory bowel disease Article Immune system medicine Humans Lymphocytes Child education education.field_of_study business.industry Innate lymphoid cell Gastroenterology Colitis Inflammatory Bowel Diseases medicine.disease Immunity Innate medicine.anatomical_structure Pediatrics Perinatology and Child Health Immunology business Dysbiosis |
| Zdroj: | J Pediatr Gastroenterol Nutr |
| ISSN: | 1536-4801 0277-2116 |
| Popis: | Objectives Very early-onset inflammatory bowel disease arises in children less than six years old, a critical time for immunologic development and maturation of the intestinal microbiome. Non-conventional lymphocytes, defined here as mucosal-associated invariant T cells and innate lymphocytes, require microbial products for either development or expansion, aspects that could be altered in very early-onset inflammatory bowel disease. Our objective was to define conventional leukocyte and non-conventional lymphocyte populations in controls and patients using multiparameter flow cytometry to test the hypothesis that their frequencies would be altered in a chronic inflammatory state associated with significant dysbiosis. Methods Multiparameter flow cytometry was used in a control cohort of 105 subjects to define age-effects, not previously comprehensively examined for these cell types in humans. Differences were defined between 263 unique age-matched patients with very early-onset inflammatory bowel disease and 105 controls using Student's t test. Subjects were divided into two age groups at the time of sampling to control for age-related changes in immune composition. Results Intermediate monocytes were consistently decreased in patients with VEO-IBD compared to controls. Mucosal-associated invariant T cells were significantly lower in patients with long-standing disease. Levels were less than half of those seen in the age-matched control cohort. The innate lymphoid cells type 2 population was expanded in the youngest patients. Conclusions Mucosal-associated invariant T cells are diminished years after presentation with inflammatory bowel disease. This durable effect of early life intestinal inflammation may have long term consequences. Diminished mucosal-associated invariant T cells could impact host defense of intestinal infections. |
| Databáze: | OpenAIRE |
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