Inhibition of HIV-1 replication by combined expression of gag dominant negative mutant and a human ribonuclease in a tightly controlled HIV-1 inducible vector
| Autor: | Crowley R, Cara A, Reitz Ms, Newton Dl, Rybak Sm, Gusella Gl, Rottschafer Se |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
viruses
Genetic enhancement Genetic Vectors Neurotoxins Clone (cell biology) Gene Products gag HIV Infections Eosinophil-Derived Neurotoxin Transfection Virus Replication medicine.disease_cause Polymerase Chain Reaction Ribonucleases Gene expression Genetics medicine Humans Ribonuclease Molecular Biology Gene Mutation Expression vector biology virus diseases rev Gene Products Human Immunodeficiency Virus Genetic Therapy Blotting Northern biology.organism_classification Virology Cell biology Gene Products rev Gene Expression Regulation Gene Products tat Lentivirus HIV-1 biology.protein Molecular Medicine tat Gene Products Human Immunodeficiency Virus HeLa Cells T-Lymphocytes Cytotoxic |
| Zdroj: | Gene Therapy. 5:65-75 |
| ISSN: | 1476-5462 0969-7128 |
| DOI: | 10.1038/sj.gt.3300545 |
| Popis: | An HIV-1-based expression vector has been constructed that produces protective genes tightly regulated by HIV-1 Tat and Rev proteins. The vector contains either a single protective gene (HIV-1 gag dominant negative mutant (delta-gag)) or a combination of two different protective genes (delta-gag and eosinophil-derived neurotoxin (EDN), a human ribonuclease) which are expressed from a dicistronic mRNA. After stable transfection of CEM T cells and following challenge with HIV-1, viral production was completely inhibited in cells transduced with the vector producing both delta-gag and EDN and delayed in cells producing delta-gag alone. In addition, cotransfection of HeLa-Tat cells with an infectious HIV-1 molecular clone and either protective vector demonstrated that the HIV-1 packaging signals present in the constructs were functional and allowed the efficient assembly of the protective RNAs into HIV-1 virions, thus potentially transmitting protection to the HIV-1 target cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|