N-butyl cyanoacrylate- and alpha-cyanoacrylate induced hepatotoxicity and venous sclerosis in a rabbit model
| Autor: | Hasan ElSayed El-Batea, Ibrahim S. Shehab El-Din, Amal A. Shehab, Asem Elfert |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis medicine.medical_treatment Haematoxylin Hemostatics Veins law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law medicine Sclerotherapy Animals Cyanoacrylates Bouin solution Fixation (histology) Sclerosis biology business.industry Pinna Gastroenterology Enbucrilate biology.organism_classification chemistry Cyanoacrylate Models Animal Lipiodol 030211 gastroenterology & hepatology Rabbits Chemical and Drug Induced Liver Injury medicine.symptom business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Arab Journal of Gastroenterology. 17:3-10 |
| ISSN: | 1687-1979 |
| DOI: | 10.1016/j.ajg.2016.01.001 |
| Popis: | Background and study aims Tissue adhesives are commonly used. The aim of this study was to assess the efficacy and hepatotoxicity of intravenous injection of N-butyl cyanoacrylate versus alpha-cyanoacrylate in a rabbit model. Materials and methods A total of 20 rabbits were divided into three groups: group I included four rabbits injected with lipiodol in the dorsal vein of a pinna (control group); group II included eight rabbits injected with N-butyl cyanoacrylate/lipiodol; and group III included eight rabbits injected with alpha-cyanoacrylate/lipiodol. All animals were left under normal living conditions for 1 week, and then euthanised. Specimens of ear and liver were taken and fixed in 10% formalin saline for histological examination. Secondary fixation was performed using Bouin solution. Specimens of ear were decalcified in ethylenediaminetetraacetic acid (EDTA) at room temperature for 3 months. Then, all specimens were processed, embedded in paraffin, sectioned, and stained with haematoxylin and eosin stains for microscopic examination. Results Microscopic examination of all specimens of the control group revealed normal structure of pinna and liver tissue. Both test groups demonstrated a wide variability of structural changes ranging from oedema and congestion to necrosis and marked cellular inflammatory infiltration. The two groups were compared using a self-designed inflammatory score. This revealed that alpha-cyanoacrylate caused more venous sclerosis with extensive perivenous reaction and hepatotoxicity than both N-butyl cyanoacrylate and control (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect