Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program

Autor: Andrew J. Henderson, Nicholas G. Martin, Busra Gok, Elliot C. Nelson, Masanao Yajima, John P. Cleary, Tanya T. Karagiannis, Christine S. Cheng
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Lipopolysaccharides
Male
Lipopolysaccharide
General Physics and Astronomy
chemistry.chemical_compound
0302 clinical medicine
Medicine
lcsh:Science
Regulation of gene expression
Multidisciplinary
Morphine
Middle Aged
medicine.anatomical_structure
Virus Diseases
Female
Single-Cell Analysis
Systems biology
medicine.drug
Adult
Science
Central nervous system
Peripheral blood mononuclear cell
Antiviral Agents
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
Young Adult
Immune system
Immunity
Humans
Transcriptomics
business.industry
Gene Expression Profiling
General Chemistry
Antimicrobial responses
Opioid-Related Disorders
Immunity
Innate
Gene expression profiling
Gene regulation in immune cells
030104 developmental biology
chemistry
Opioid
Gene Expression Regulation
Immunology
Leukocytes
Mononuclear
lcsh:Q
Interferons
business
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-020-16159-y
Popis: Chronic opioid usage not only causes addiction behavior through the central nervous system, but also modulates the peripheral immune system. However, how opioid impacts the immune system is still barely characterized systematically. In order to understand the immune modulatory effect of opioids in an unbiased way, here we perform single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from opioid-dependent individuals and controls to show that chronic opioid usage evokes widespread suppression of antiviral gene program in naive monocytes, as well as in multiple immune cell types upon stimulation with the pathogen component lipopolysaccharide. Furthermore, scRNA-seq reveals the same phenomenon after a short in vitro morphine treatment. These findings indicate that both acute and chronic opioid exposure may be harmful to our immune system by suppressing the antiviral gene program. Our results suggest that further characterization of the immune modulatory effects of opioid is critical to ensure the safety of clinical opioids.
Over 100 million of opioid prescriptions are issued yearly in the USA alone, but the impact of opioid use on the immune system is barely characterized. Here the authors report antiviral immune response is blunted in several types of blood cells from opioid-dependent individuals, and when healthy donor cells are exposed to morphine in a dish.
Databáze: OpenAIRE
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