Identification of the binding site of two monoclonal antibodies to human protamine
| Autor: | Rod Balhorn, Andrew J. Wyrobek, Larry H. Stanker, Cathy. McKeown |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Antigenicity medicine.drug_class Immunology Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Rodentia Cross Reactions Monoclonal antibody Epitope Epitopes Mice Antibody Specificity Cricetinae medicine Animals Humans Amino Acid Sequence Protamines Nuclear protein Binding site Molecular Biology Artiodactyla chemistry.chemical_classification biology Sequence Homology Amino Acid Antibodies Monoclonal Haplorhini Protamine Molecular biology Peptide Fragments Amino acid Rats chemistry Biochemistry biology.protein Cattle Rabbits Antibody |
| Zdroj: | Molecular immunology. 30(18) |
| ISSN: | 0161-5890 |
| Popis: | We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to the evolutionarily conservative ammo-terminal region of protamine 1. This assignment is based ob antibody binding to protamine from different species in which the protamine sequence is known, as well as analysis of antibody binding to synthetic peptides and synthetic peptides with specific amino acid substitutions. |
| Databáze: | OpenAIRE |
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