Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI
| Autor: | Vânia D'Almeida, Renata B. Oliveira, Beatriz Jurkiewcz Frangipani, Sandra Obikawa Kyosen, Vaneisse Cristina Lima Monteiro, Joyce Umbelino Pinto Yamamoto, José Araújo de Oliveira-Silva, Ana Maria Martins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.medical_specialty lcsh:QH426-470 Mucopolysaccharidosis chemistry.chemical_element Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Internal medicine Mucopolysaccharidosis I Genetics medicine oxidative stress In patient selenium glutathione peroxidase Molecular Biology chemistry.chemical_classification Glutathione peroxidase Glutathione Enzyme replacement therapy medicine.disease lcsh:Genetics 030104 developmental biology Endocrinology chemistry Human and Medical Genetics Selenium Oxidative stress 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Biology v.42 n.1 2019 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Issue: ahead, Published: 14 FEB 2019 Genetics and Molecular Biology, Volume: 42, Issue: 1, Pages: 1-8, Published: 14 FEB 2019 Genetics and Molecular Biology, Vol 42, Iss 1, Pp 1-8 (2019) |
| Popis: | We assessed levels of plasma selenium (Se), selenoproteins and their change after Se supplementation in patients with mucopolysaccharidosis (MPS) types I, II and VI. This was done in a retrospective study of the medical records of 30 patients with MPS I (n=13), MPS II (n=9) and MPS VI (n=8) who were being treated with enzyme replacement therapy. As part of routine nutritional monitoring, Se levels were measured, revealing that 28 patients (93.3%) had values below the normal range. Therefore, they received supplementation for 12 months, and Se was measured after 6 and 12 months. Glutathione peroxidase (GPx) activity, total glutathione (GSHt), oxidized glutathione (GSSG) and reduced glutathione (GSH) were measured at baseline and 6 months after Se supplementation. The mean GSHt at baseline was 7.90 ± 2.36 μmol/g Hb, and after Se supplementation it was 5.76 ± 1.13 μmol/g Hb; GSH/GSSG was 2.3 ± 1.16 at baseline and 0.58 ± 0.38 after supplementation. GPx activity was 16.46 ± 3.31 U/g Hb at baseline and 4.53 ± 4.92 U/g Hb after Se supplementation. The difference was shown to be statistically significant by paired t-test. In conclusion, our study demonstrated that oxidative stress parameters were altered by Se supplementation in patients with MPS I, II and VI who were previously deficient in Se. |
| Databáze: | OpenAIRE |
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