Peptide-protein microarrays for the simultaneous detection of pathogen infections
| Autor: | Frédéric Malingue, Hélène Gras-Masse, Ahmed Bouzidi, Rémi Desmet, Fenhling Zhou, Christophe Olivier, Xavier Duburcq, Oleg Melnyk, Claude Auriault |
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| Rok vydání: | 2004 |
| Předmět: |
Epstein-Barr Virus Infections
Herpesvirus 4 Human viruses Hepatitis C virus Biomedical Engineering Protein Array Analysis Pharmaceutical Science Bioengineering medicine.disease_cause Immunofluorescence Gp41 Communicable Diseases Virus Hepatitis Antigen Hepatitis Viruses medicine Humans Serologic Tests Treponema pallidum Pharmacology Hepatitis B virus NS3 medicine.diagnostic_test Chemistry Organic Chemistry virus diseases Virology Molecular biology digestive system diseases Protein microarray Peptides Biotechnology |
| Zdroj: | Bioconjugate chemistry. 15(2) |
| ISSN: | 1043-1802 |
| Popis: | We describe novel peptide−protein microarrays, which were fabricated using semicarbazide glass slides that permitted the immobilization of glyoxylyl peptides by site-specific ligation and the immobilization of proteins by physisorption. The arrays permitted the simultaneous serodetection of antibodies directed against hepatitis C virus (HCV core p21 15−45 peptide, NS4 1925−1947 peptide, core, NS3, NS4, and mixture of core, NS3, NS4, and NS5 antigens), hepatitis B virus (HBc, HBe, and HBs), human immunodeficiency virus (Gp41 and Gp120 for HIV-I and Gp36 for HIV-II), Epstein−Barr virus (VCAp18 153-176 peptide), and syphilis (rTpN47 and rTpN17) antigens using an immunofluorescence assay. Peptide−protein microarrays displayed high signal-to-noise ratios, sensitivities, and specificities for the detection of antibodies as revealed by the analysis of a collection of human sera referenced against these five pathogens. |
| Databáze: | OpenAIRE |
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