Bioinformatics analysis and verification of gene targets for benign tracheal stenosis
| Autor: | Lining Huang, Wenhao Wu, Yong Qiu, Ling‐bing Meng, Wen‐ping Song, Pei Zhang, Wei Yang, Shuxian Ma, Xuze Li, Zi-chen Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine differentially expressed genes lcsh:QH426-470 Cell division 030105 genetics & heredity Biology 03 medical and health sciences Genetics medicine Humans Gene Regulatory Networks Cyclin B2 Cyclin B1 tracheal stenosis KEGG Molecular Biology Gene Genetics (clinical) Aged Aged 80 and over bioinformatic Computational Biology Mitotic nuclear division Original Articles Middle Aged Cell cycle Hyperplasia medicine.disease Tracheal Stenosis lcsh:Genetics Ki-67 Antigen 030104 developmental biology Female Original Article cell cycle Transcriptome Toxicogenomics |
| Zdroj: | Molecular Genetics & Genomic Medicine, Vol 8, Iss 6, Pp n/a-n/a (2020) Molecular Genetics & Genomic Medicine |
| ISSN: | 2324-9269 |
| Popis: | Background Tracheal injury could cause intratracheal scar hyperplasia which in turn causes benign tracheal stenosis (TS). With the increasing use of mechanical ventilation and ventilator, the incidence of TS is increasing. However, the molecular mechanisms of TS have not been elucidated. It is significant to further explore the molecular mechanisms of TS. Methods The repeatability of public data was verified. Differently expressed genes (DEGs) and most significant genes were identified between TS and normal samples. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed. The comparative toxicogenomics database were analyzed. TS patients were recruited and RT‐qPCR were performed to verify the most significant genes. Results There exist strong correlations among samples of TS and normal group. There was a total of 194 DEGs, including 61 downregulated DEGs and 133 upregulated DEGs. GO were significantly enriched in mitotic nuclear division, cell cycle, and cell division. Analysis of KEGG indicated that the top pathways were cell cycle, and p53 pathway. MKI67(OMIM:176741), CCNB1(OMIM:123836), and CCNB2(OMIM:602755) were identified as the most significant genes of TS, and validated by the clinical samples. Conclusion Bioinformatics methods might be useful method to explore the mechanisms of TS. In addition, MKI67, CCNB1, and CCNB2 might be the most significant genes of TS. Bioinformatics methods could be useful tools to predict the progression of tracheal stenosis (TS) and to explore the mechanisms of the occurrence and development of TS. In addition, there are differently expressed genes (DEGs) between TS and normal tracheal tissues, especially MKI67, CCNB1, and CCNB2. |
| Databáze: | OpenAIRE |
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