Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation
Autor: | Marcus Vinicius Pereira dos Santos Nascimento, Fábio Arruda-Silva, Ana Beatriz Gobbo Luz, Eduardo Monguilhott Dalmarco, Gustavo S. Queiroz, Moacir Geraldo Pizzolatti, Tânia Silvia Fröde, Dalila Venzke |
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Rok vydání: | 2014 |
Předmět: |
Polygala
Adenosine Deaminase Interleukin-1beta Immunology Anti-Inflammatory Agents Inflammation Carrageenan Nitric Oxide Leukocyte Count Mice Rutin chemistry.chemical_compound medicine Animals Immunology and Allergy RNA Messenger Pleurisy Peroxidase Pharmacology Traditional medicine biology Plant Extracts Tumor Necrosis Factor-alpha business.industry NF-kappa B medicine.disease biology.organism_classification Chemotaxis Leukocyte chemistry Polygalaceae medicine.symptom Nf κb activation business Phytotherapy |
Zdroj: | International Immunopharmacology. 19:334-341 |
ISSN: | 1567-5769 |
DOI: | 10.1016/j.intimp.2014.02.010 |
Popis: | This study was conducted to investigate the anti-inflammatory activity of Polygala molluginifolia (Polygalaceae) on the mouse pleurisy model induced by carrageenan. P. molluginifolia is a plant native to southern Brazil that is popularly called "canfora". The Polygala genus is used to treat different pathologies, including inflammatory diseases, in traditional medicine.The whole P. molluginifolia plant material was extracted by maceration with 96% ethanol. The crude hydroalcoholic extract (CE) was subjected to chromatographic procedures to produce various derivate fractions, including its aqueous (Aq), ethyl acetate (EtOAc), and hexane (Hex) fractions. Compound 1 (5,3',4'-trihydroxy-6″,6″-dimethylpyrano [2″,3″:7,6] isoflavone) (Iso), which was isolated from the EtOAc fraction, and Compound 2 (rutin) (Rut), which was isolated from the Aq fraction, were identified using ¹H and ¹³C NMR spectroscopy and quantified using an HPLC apparatus.The CE, the Aq, EtOAc, and Hex fractions, and the isolated compounds Iso and Rut were able to reduce cell migration and exudation. Furthermore, the plant material also decreased the myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities and the nitric oxide (NO(x)), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) levels. In addition, Iso and Rut reduced the TNF-α and IL-1β mRNA expression levels and significantly decreased NF-κB p65 phosphorylation.The results show that P. molluginifolia has a significant anti-inflammatory action and that this effect is due, at least in part, to the presence of Iso and Rut in large amounts. Moreover, this effect was found to be closely related to the inhibitory effects of the isolated compounds on the NF-κB pathway. |
Databáze: | OpenAIRE |
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