Organic matter reduces the amount of detectable environmental DNA in freshwater
| Autor: | Freek T. Bakker, Kees van Bochove, Rutger A. Vos, Barbara Gravendeel, Kevin K. Beentjes, Lia Hemerik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Amphipoda detection Wiskundige en Statistische Methoden - Biometris 010603 evolutionary biology 01 natural sciences Population density 03 medical and health sciences Gammarus pulex lcsh:QH540-549.5 Organic matter Environmental DNA Mathematical and Statistical Methods - Biometris Ecology Evolution Behavior and Systematics Organism Original Research 030304 developmental biology Nature and Landscape Conservation degradation organic matter chemistry.chemical_classification 0303 health sciences Ecology biology pH Plant Ecology PE&RC biology.organism_classification environmental DNA Biosystematiek chemistry Water temperature Environmental chemistry Biosystematics lcsh:Ecology EPS |
| Zdroj: | Ecology and Evolution, Vol 10, Iss 8, Pp 3647-3654 (2020) Ecology and Evolution 10 (2020) 8 Ecology and Evolution Ecology and Evolution, 10, 3647-3654 Ecology and Evolution, 10, 8, pp. 3647-3654 Ecology and Evolution, 10(8), 3647-3654 |
| ISSN: | 2045-7758 |
| Popis: | Environmental DNA (eDNA) is used for monitoring the occurrence of freshwater organisms. Various studies show a relation between the amount of eDNA detected and target organism abundance, thus providing a potential proxy for reconstructing population densities. However, environmental factors such as water temperature and microbial activity are known to affect the amount of eDNA present as well. In this study, we use controlled aquarium experiments using Gammarus pulex L. (Amphipoda) to investigate the relationship between the amount of detectable eDNA through time, pH, and levels of organic material. We found eDNA to degrade faster when organic material was added to the aquarium water, but that pH had no significant effect. We infer that eDNA contained inside cells and mitochondria is extra resilient against degradation, though this may not reflect actual presence of target species. These results indicate that, although estimation of population density might be possible using eDNA, measured eDNA concentration could, in the future, be corrected for local environmental conditions in order to ensure accurate comparisons. We found eDNA to degrade faster when organic material was added to the aquarium water, but that pH had no significant effect. Results indicate that, although estimation of population density might be possible using eDNA, measured eDNA concentration could, in the future, be corrected for local environmental conditions in order to ensure accurate comparisons. |
| Databáze: | OpenAIRE |
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