Transcriptomics and immunological analyses reveal a pro-angiogenic and anti-inflammatory phenotype for decidual endothelial cells
| Autor: | E. Masat, Marie-Pierre Piccinni, Alessandro Mangogna, Massimo Degan, Renzo Menegazzi, Valter Gattei, Uday Kishore, Gabriella Zito, Roberta Bulla, Chiara Agostinis, Fleur Bossi, Letizia Lombardelli, Giuseppe Ricci |
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| Přispěvatelé: | Agostinis, C., Masat, E., Bossi, F., Ricci, G., Menegazzi, R., Lombardelli, L., Zito, G., Mangogna, A., Degan, M., Gattei, V., Piccinni, M. -P., Kishore, U., Bulla, R. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chemokine Leukocyte migration Angiogenesis Fluorescent Antibody Technique Chemokine CXCL9 lcsh:Chemistry angiogenesis 0302 clinical medicine Pregnancy Decidua Endothelium Inflammation Skin lcsh:QH301-705.5 Spectroscopy Cells Cultured Endothelial Cell Cultured 030219 obstetrics & reproductive medicine biology Neovascularization Pathologic Cell adhesion molecule Chemistry Reverse Transcriptase Polymerase Chain Reaction General Medicine Intercellular Adhesion Molecule-3 CD Computer Science Applications Cell biology Angiogenesi Tumor necrosis factor alpha Female medicine.symptom Human decidua skin Antigens CD Cell Adhesion Molecules Chemokine CXCL10 Endothelial Cells Humans In Vitro Techniques Interferon-gamma Tumor Necrosis Factor-alpha endothelium Cells Catalysis Article Inorganic Chemistry 03 medical and health sciences Immune system medicine CXCL10 Antigens Physical and Theoretical Chemistry Molecular Biology Neovascularization Pathologic In Vitro Technique Organic Chemistry 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Cell Adhesion Molecule inflammation biology.protein |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 20, Iss 7, p 1604 (2019) Volume 20 Issue 7 |
| Popis: | Background: In pregnancy, excessive inflammation and break down of immunologic tolerance can contribute to miscarriage. Endothelial cells (ECs) are able to orchestrate the inflammatory processes by secreting pro-inflammatory mediators and bactericidal factors by modulating leakiness and leukocyte trafficking, via the expression of adhesion molecules and chemokines. The aim of this study was to analyse the differences in the phenotype between microvascular ECs isolated from decidua (DECs) and ECs isolated from human skin (ADMECs). Methods: DECs and ADMECs were characterized for their basal expression of angiogenic factors and adhesion molecules. A range of immunological responses was evaluated, such as vessel leakage, reactive oxygen species (ROS) production in response to TNF-&alpha stimulation, adhesion molecules expression and leukocyte migration in response to TNF-&alpha and IFN-&gamma stimulation. Results: DECs produced higher levels of HGF, VEGF-A and IGFBP3 compared to ADMECs. DECs expressed adhesion molecules, ICAM-2 and ICAM-3, and a mild response to TNF-&alpha was observed. Finally, DECs produced high levels of CXCL9/MIG and CXCL10/IP-10 in response to IFN-&gamma and selectively recruited Treg lymphocytes. Conclusion: DEC phenotype differs considerably from that of ADMECs, suggesting that DECs may play an active role in the control of immune response and angiogenesis at the foetal-maternal interface. |
| Databáze: | OpenAIRE |
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