CD31 (PECAM-1) Serves as the Endothelial Cell-Specific Receptor of Clostridium perfringens β-Toxin
| Autor: | Basma Tarek, Christoph von Ballmoos, Julia Bruggisser, Horst Posthaus, Guillaume Witz, Britta Engelhardt, Gaby Enzmann, Urban Deutsch, Philipp Müller, Marianne Wyder |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CD31
Male Endothelium Clostridium perfringens Swine Virulence Factors Bacterial Toxins Biology medicine.disease_cause Microbiology Virulence factor Cell Line 03 medical and health sciences Mice 0302 clinical medicine 510 Mathematics Cell surface receptor Virology 540 Chemistry Extracellular medicine Animals Humans Protein Interaction Domains and Motifs Amino Acid Sequence Receptor 610 Medicine & health Cells Cultured 030304 developmental biology Mice Knockout 0303 health sciences 630 Agriculture Endothelial Cells Molecular biology Endothelial stem cell Mice Inbred C57BL Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure surgical procedures operative Clostridium Infections cardiovascular system 570 Life sciences biology Parasitology Female 030217 neurology & neurosurgery circulatory and respiratory physiology |
| DOI: | 10.7892/boris.145610 |
| Popis: | Clostridium perfringens β-toxin (CPB) is a highly active β-pore-forming toxin (β-PFT) and the essential virulence factor for fatal, necro-hemorrhagic enteritis in animals and humans. The molecular mechanisms involved in CPB's action on its target, the endothelium of small intestinal vessels, are poorly understood. Here, we identify platelet endothelial cell adhesion molecule-1 (CD31 or PECAM-1) as the specific membrane receptor for CPB on endothelial cells. CD31 expression corresponds with the cell-type specificity of CPB, and it is essential for toxicity in cultured cells and mice. Ectopic CD31 expression renders resistant cells and liposomes susceptible to CPB-induced membrane damage. Moreover, the extracellular Ig6 domain of mouse, human, and porcine CD31 is essential for the interaction with CPB. Hence, our results explain the cell-type specificity of CPB in vitro and in the natural disease caused by C. perfringens type C. |
| Databáze: | OpenAIRE |
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