Investigation of means to improve rates of fertilization in in vitro matured/in vitro fertilized bovine oocytes
| Autor: | N.D. Nel, Robert J. Wall, H. W. Hawk, R.A. Waterman |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
endocrine system
urogenital system Equine Chemistry Embryo culture Cleavage (embryo) In vitro Andrology Human fertilization Food Animals Hyaluronidase medicine Animal Science and Zoology Small Animals hormones hormone substitutes and hormone antagonists reproductive and urinary physiology medicine.drug |
| Zdroj: | Theriogenology. 38(6) |
| ISSN: | 0093-691X |
| Popis: | Experiments were conducted with 5,979 oocytes to determine whether detaching some of the cumulus cells from oocytes either before or after maturation would improve the fertilization rate and proportion of oocytes that developed to expanded blastocysts. Oocytes were aspirated from ovaries of slaughtered cows and matured, fertilized and cultured in vitro. Pipetting immature oocytes before maturation to detach some of the cumulus, with all cumulus cells left in the maturation wells, significantly increased fertilization rates, especially of oocytes that initially had a full cumulus investment. In further experiments, pipetting oocytes either before or after maturation to detach most of the cumulus, or treating with hyaluronidase after maturation to disperse the cumulus, significantly increased fertilization rates and proportions of oocytes developing to expanded blastocysts. |
| Databáze: | OpenAIRE |
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