ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort
| Autor: | Romina Storb, Juan Cabrera, Elisabet Aranda, Sebastián Fracchia, Santiago A Varela, Javier E. Moreno, Maya Svriz, Inés Ponce de León, Nahuel Spinedi, Facundo Romani, Ramón Alberto Batista-García, J Martín Scervino |
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| Přispěvatelé: | Aranda, Elisabet [0000-0001-5915-2445], Romani, Facundo [0000-0003-3954-6740], Moreno, Javier E. [0000-0001-9763-5325], Batista-García, Ramón Alberto [0000-0002-1032-0767], Ponce de León, Inés [0000-0001-6495-4055], Scervino, J. Martín [0000-0003-2631-3497], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Liverwort Antioxidant medicine.medical_treatment polycyclic aromatic hydrocarbons RNA-sequencing Plant Science Photosynthesis 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Marchantia polymorpha Downregulation and upregulation liverwort antioxidant enzymes medicine Ecology Evolution Behavior and Systematics chemistry.chemical_classification Anthracene photosynthesis Ecology biology Botany anthracene biology.organism_classification Polycyclic aromatic hydrocarbons Chloroplast 030104 developmental biology Enzyme chemistry Biochemistry Peroxidases QK1-989 biology.protein Antioxidant enzymes peroxidases 010606 plant biology & botany Peroxidase |
| Zdroj: | Plants, Vol 10, Iss 1478, p 1478 (2021) Plants Digibug. Repositorio Institucional de la Universidad de Granada instname Volume 10 Issue 7 |
| ISSN: | 2223-7747 |
| Popis: | This study was financed by CONICET Argentina (PIP0235), Comahue-University (PIN II 04B216), Agencia I+D+i (PICT2017-1484, PICT2018-00650, PICT-2019-00073) and "Fondo Conjunto Uruguay-Mexico" (AUCI-AMEXCID). EA thanks the Ministry of Economy and Competitiveness and the ERDF for co-funding through the "Ramon y Cajal Contract" (grant number RYC-2013-12481). Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl a and 38.4% of Chl b, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (Fv/Fm). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that M. polymorpha tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that M. polymorpha under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus. Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) PIP0235 Comahue-University PIN II 04B216 Agencia I+D+i PICT2017-1484 PICT2018-00650 PICT-2019-00073 "Fondo Conjunto Uruguay-Mexico" (AUCI-AMEXCID) European Commission RYC-2013-12481 Ministry of Economy and Competitiveness RYC-2013-12481 |
| Databáze: | OpenAIRE |
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