Discrimination of cancerous and healthy colon tissues: A new laser-based method
| Autor: | Mustafa Sahin, Hamdi Şükür Kiliç, Yasemin Gündoğdu, Pınar Karabağlı, Hüsnü Alptekin |
|---|---|
| Přispěvatelé: | Selçuk Üniversitesi, Fen Fakültesi, Fizik Bölümü, Gündoğdu, Yasemin., Alptekin, Hüsnü., Karabağlı, Pınar., Şahin, Mustafa., Kilic, Hamdi S. |
| Rok vydání: | 2018 |
| Předmět: |
colon tissue
tumor Materials science Colon medicine.medical_treatment Dermatology Adenocarcinoma Mass spectrometry 01 natural sciences law.invention 010309 optics Diagnosis Differential 030207 dermatology & venereal diseases 03 medical and health sciences fs-LIMS 0302 clinical medicine law 0103 physical sciences medicine Humans Oscilloscope nontumor PCA Principal Component Analysis Lasers Ablation Laser Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Principal component analysis Femtosecond Colonic Neoplasms Mass spectrum Surgery Ultrashort pulse Biomedical engineering |
| Zdroj: | Lasers in surgery and medicine. 51(4) |
| ISSN: | 1096-9101 3057-5060 |
| Popis: | WOS: 000465344200009 PubMed: 30575060 Background Femtosecond (fs) Laser Ionisation Mass Spectrometry (fs-LIMS) on colon tissues are described and investigated using ionization/fragmentation processes in details to present a new application in this study. Linear Time of Flight (L-TOF) mass analyzer was utilized to investigate paraffin-embedded human tissue in this study. The effect of fs laser intensity on the spectral characteristics was investigated and interpreted due to mass spectra obtained using 800 nm wavelength with 90 fs pulses at 1 kHz repetition rate. Objectives Mass spectra of tissues were recorded from L-TOF system and then analyzed by performing a statistical approach called Principal Component Analysis (PCA). The fs-LIMS method applied is proposed as a new and pioneering technology to analyze tissues using L-TOF system, as a human free fast and reliable intra-operative cancer diagnosis method for guiding surgeon to clean the edges of cancerous tissues to be applied during the surgical operation, for pathological examinations. Fs-LIMS provides some unique diagnosis opportunities to investigate biochemical characteristics of cancerous tissues leading to obtain sensitive, fast, and reliable results. The analysis of tissue is based on distribution of molecular ion (m/z) peaks in low mass region ( Scientific Research Projects Coordination Unit of Selcuk UniversitySelcuk University [15201096] Contract grant sponsor: Scientific Research Projects Coordination Unit of Selcuk University; Contract grant numbers: 15201096. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text