Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D
Autor: | Barkha Sharma, Saurabh Gangola, Saurabh Kumar, Samiksha Joshi, Anita Sharma |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Proteomics
Applied Microbiology Protein Expression Bacillus cereus Laccases Bacillus Pathology and Laboratory Medicine High-performance liquid chromatography Biochemistry Mass Spectrometry Cypermethrin Analytical Chemistry chemistry.chemical_compound Spectrum Analysis Techniques Agricultural Soil Science Pyrethrins Medicine and Health Sciences Fipronil Bacillus Cereus Chromatography High Pressure Liquid Multidisciplinary biology Chromatographic Techniques Agriculture Enzymes Bacterial Pathogens Chemistry Medical Microbiology Physical Sciences Biodegradation Medicine Engineering and Technology Pathogens Agrochemicals Research Article Biotechnology Science Soil Science Bioengineering Research and Analysis Methods Microbiology Gas Chromatography-Mass Spectrometry Environmental Biotechnology Bacterial Proteins Imidacloprid Stress Physiological Gene Expression and Vector Techniques Pesticides Molecular Biology Techniques Microbial Pathogens Molecular Biology Molecular Biology Assays and Analysis Techniques Chromatography Bacteria Organisms Biology and Life Sciences Proteins Pesticide biology.organism_classification chemistry Enzymology Earth Sciences Gas chromatography Pest Control |
Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 8, p e0253106 (2021) |
ISSN: | 1932-6203 |
Popis: | A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in gas chromatography mass spectrometry (GC-MS) analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (Accession ID: MH341691). Stress responsive and catabolic/pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzymatic kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions which suggested that the production of enzyme was induced significantly in pesticide stress (163 μg.μL-1) as compare to normal conditions(29 μg.μL-1) while the Km value was decreased in pesticides stress condition (Km = 10.57 mM) and increases in normal condition (Km = 14.33 mM).Amplification of laccase gene showed a major band of 1200bp. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water). |
Databáze: | OpenAIRE |
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