Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D

Autor: Barkha Sharma, Saurabh Gangola, Saurabh Kumar, Samiksha Joshi, Anita Sharma
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Proteomics
Applied Microbiology
Protein Expression
Bacillus cereus
Laccases
Bacillus
Pathology and Laboratory Medicine
High-performance liquid chromatography
Biochemistry
Mass Spectrometry
Cypermethrin
Analytical Chemistry
chemistry.chemical_compound
Spectrum Analysis Techniques
Agricultural Soil Science
Pyrethrins
Medicine and Health Sciences
Fipronil
Bacillus Cereus
Chromatography
High Pressure Liquid
Multidisciplinary
biology
Chromatographic Techniques
Agriculture
Enzymes
Bacterial Pathogens
Chemistry
Medical Microbiology
Physical Sciences
Biodegradation
Medicine
Engineering and Technology
Pathogens
Agrochemicals
Research Article
Biotechnology
Science
Soil Science
Bioengineering
Research and Analysis Methods
Microbiology
Gas Chromatography-Mass Spectrometry
Environmental Biotechnology
Bacterial Proteins
Imidacloprid
Stress
Physiological
Gene Expression and Vector Techniques
Pesticides
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Molecular Biology Assays and Analysis Techniques
Chromatography
Bacteria
Organisms
Biology and Life Sciences
Proteins
Pesticide
biology.organism_classification
chemistry
Enzymology
Earth Sciences
Gas chromatography
Pest Control
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 8, p e0253106 (2021)
ISSN: 1932-6203
Popis: A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in gas chromatography mass spectrometry (GC-MS) analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (Accession ID: MH341691). Stress responsive and catabolic/pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzymatic kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions which suggested that the production of enzyme was induced significantly in pesticide stress (163 μg.μL-1) as compare to normal conditions(29 μg.μL-1) while the Km value was decreased in pesticides stress condition (Km = 10.57 mM) and increases in normal condition (Km = 14.33 mM).Amplification of laccase gene showed a major band of 1200bp. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).
Databáze: OpenAIRE
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