Exploiting biosynthetic gold nanoparticles for improving the aqueous solubility of metal-free phthalocyanine as biocompatible PDT agent
| Autor: | M. A. Harith, Rehab M. Amin, Mahmoud A. Sliem, Ragaa M. EL-Balshy, Shaimaa Alexeree |
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| Rok vydání: | 2017 |
| Předmět: |
Indoles
Materials science Biocompatibility Metal Nanoparticles Infrared spectroscopy Bioengineering 02 engineering and technology Isoindoles Conjugated system 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Materials Testing Organic chemistry Fourier transform infrared spectroscopy technology industry and agriculture 021001 nanoscience & nanotechnology 0104 chemical sciences Photochemotherapy Solubility chemistry Mechanics of Materials Colloidal gold Phthalocyanine Amine gas treating Gold 0210 nano-technology Nanoconjugates Nuclear chemistry |
| Zdroj: | Materials Science and Engineering: C. 76:727-734 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2017.03.129 |
| Popis: | Increasing the limit of dispersion of metal-free phthalocyanine (H2Pc) in an aqueous medium using biosynthetic gold nanoparticles for photodynamic therapy (PDT) is investigated. Gold nanoparticles (Au NPs) are biosynthesized in one step using Potatoes (Solanum tuberosum) extract and are characterized by UV/VIS spectrophotometry, Fourier transformer infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The metal-free phthalocyanine is conjugated to the surface of the gold nanoparticles in a side to side regime through the secondary amine groups of H2Pc. The clear violet solution of phthalocyanine-gold (Pc-Au) nanoconjugates is investigated by UV–VIS, FTIR and TEM techniques. Disappearance of the absorption band of the secondary amine in the Pc-Au nanoconjugates compared to that of the parent H2Pc, and detection of the absorption band of H2Pc in the aqueous medium confirmed the dispersion of H2Pc and consequently the loading of H2Pc on the surface of Au NPs. The cytotoxic effect of biosynthetic gold nanoparticles and Pc-Au nanoconjugates compared to chemically synthesized gold nanoparticles on buffalo epithelial cells has been studied in vitro. Interestingly, the results showed that the biosynthetic Au NPs as well as Pc-Au nanoconjugates have no effect on buffalo epithelial cells viability, which indicating their biocompatibility contrary to the chemically synthesized Au NPs. This work will open the door, for the first time, for using H2Pc suspended in water for PDT and other phototherapeutic applications. |
| Databáze: | OpenAIRE |
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