| Autor: |
Pf��ffle, Clara, Hillmann, Dierck, Spahr, Hendrik, Kutzner, Lisa, Burhan, Sazan, Hilge, Felix, Miura, Yoko, H��ttmann, Gereon |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
Imaging neuronal activity non-invasively in vivo is of tremendous interest, but current imaging techniques lack either functional contrast or necessary microscopic resolution. The retina is the only part of the central nervous system (CNS) that allows us direct optical access. Not only ophthalmic diseases, but also many degenerative disorders of the CNS go along with pathological changes in the retina. Consequently, functional analysis of retinal neurons could lead to an earlier and better diagnosis and understanding of those diseases. Recently, we showed that an activation of photoreceptor cells could be visualized in humans using a phase sensitive evaluation of optical coherence tomography data. The optical path length of the outer segments changes by a few hundred nanometers in response to optical stimulation. Here, we show simultaneous imaging of the activation of photoreceptor and ganglion cells. The signals from the ganglion cells are ten-fold smaller than those from the photoreceptor cells and were only visible using new algorithms for suppressing motion artifacts. This allowed us to generate a wiring diagram showing functional connections between photoreceptors and ganglion cells. We present a theoretical model that explains the observed intrinsic optical signals by osmotic volume changes, induced by ion influx or efflux. Since all neuronal activity is associated with ion fluxes, imaging osmotic induced size changes with nanometer precision should visualize activation in any neuron. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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