Neutrophils negatively regulate induction of mucosal IgA responses after sublingual immunization
| Autor: | Tara L. Martin, Estelle Cormet-Boyaka, Astrid Bonnegarde-Bernard, Yoichiro Iwakura, Junbae Jee, Haley Steiner, Ryan C. Bachman, Alexandra Duverger, Prosper N. Boyaka |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Neutrophils
animal diseases Immunology Bacillus anthracis edema toxin IKKβ Bacterial Toxins Administration Sublingual chemical and pharmacologic phenomena Mice Transgenic Biology Article 03 medical and health sciences Leukocyte Count Mice 0302 clinical medicine medicine Immunology and Allergy Animals Myeloid Cells Mucosal iga Immunity Mucosal 030304 developmental biology 0303 health sciences Microbial toxins Antigens Bacterial B-Lymphocytes Mucous Membrane I-Kappa-B Kinase Mucous membrane vaccine adjuvant Immunoglobulin A.secretory biochemical phenomena metabolism and nutrition 3. Good health I-kappa B Kinase medicine.anatomical_structure Immunization Neutrophil Infiltration Antibody Formation Immunoglobulin A Secretory bacteria Mucosal IgA Lymph Nodes Signal transduction Antibody formation 030215 immunology Signal Transduction |
| Zdroj: | Mucosal immunology |
| ISSN: | 1935-3456 1933-0219 |
| Popis: | Induction of mucosal immunoglobulin-A (IgA) capable of providing a first line of defense against bacterial and viral pathogens remains a major goal of needle-free vaccines given via mucosal routes. Innate immune cells are known to play a central role in induction of IgA responses by mucosal vaccines, but the relative contribution of myeloid cell subsets to these responses has not firmly been established. Using an in vivo model of sublingual vaccination with Bacillus anthracis edema toxin (EdTx) as adjuvant, we examined the role of myeloid cell subsets for mucosal secretory IgA responses. Sublingual immunization of wild-type mice resulted in a transient increase of neutrophils in sublingual tissues and cervical lymph nodes. These mice later developed Ag-specific serum IgG responses, but not serum or mucosal IgA. Interestingly, EdTx failed to increase neutrophils in sublingual tissues and cervical lymph nodes of IKKβ(ΔMye) mice, and these mice developed IgA responses. Partial depletion of neutrophils before immunization of wild-type mice allowed the development of both mucosal and serum IgA responses. Finally, co-culture of B cells with neutrophils from either wild-type or IKKβ(ΔMye) mice suppressed secretion of IgA, but not IgM or IgG. These results identify a new role for neutrophils as negative regulators of IgA responses. |
| Databáze: | OpenAIRE |
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