Embryonic expression pattern of the Drosophila Kallmann syndrome gene kal-1
| Autor: | Elena I. Rugarli, Maria Rosaria Grimaldi, Davide Andrenacci, Franco Graziani, Stéphanie Le Bras |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Whole-mount in situ hybridization Gonad Kallmann syndrome Somatic cell Organogenesis Biology Anosmin-1 Genetics medicine Animals Drosophila Proteins Gonads kal-1 Molecular Biology In Situ Hybridization Extracellular Matrix Proteins Kallmann Syndrome medicine.disease Head involution Immunohistochemistry Dorsal closure Cell biology Chordotonal organ Smell Development Drosophila medicine.anatomical_structure biology.protein Female Deformed Homeotic gene Biomarkers Kallmann syndrom Developmental Biology |
| Zdroj: | Gene expression patterns (Tokyo) 5 (2004): 67–73. doi:10.1016/j.modgep.2004.06.004 info:cnr-pdr/source/autori:Andrenacci D, Le Bras S, Grimaldi MR, Rugarli E, Graziani F/titolo:Embryonic expression pattern of the Drosophila Kallmann syndrome gene kal-1/doi:10.1016%2Fj.modgep.2004.06.004/rivista:Gene expression patterns (Tokyo)/anno:2004/pagina_da:67/pagina_a:73/intervallo_pagine:67–73/volume:5 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.modgep.2004.06.004 |
| Popis: | The role of kal-1, the gene responsible for the X chromosome-linked form of Kallmann syndrome, is not well definite. In Drosophila, the kal-1 gene encodes a putative protein with the characteristic kal-1 topology but with only two Fibronectin-like type III (FnIII) domains. We studied the embryonic expression pattern of kal-1 using whole mount in situ hybridization. This gene is expressed in the second half of embryogenesis showing a complex and dynamic pattern. kal-1 is expressed during important morphogenetic processes such as germ band retraction, dorsal closure and head involution. We found expression in cells associated with different sensory organs, such as the antennal organ, which has an olfactory function, the chordotonal organ, the Keilin's organ and the dorsal pharyngeal organ. Expression of kal-1 in the head also regards some ectodermal cells of the gnathal lobes. By studying the expression in Dfd and cnc homeotic mutants, we found that these ectodermal cells derive from the anterior and posterior mandibular segment, whose determination depends on cnc, and that the expression in the posterior mandibular segment requires Dfd activity. kal-1 is also expressed in the posterior part of the male gonads in a specific subset of the somatic cells called male-specific somatic gonadal precursors (msSGPs). This is the first time that the expression of a kal-1 ortholog has been demonstrated to be sex specific making the kal-1 transcript a useful tool for the study of sex determination in the gonad. |
| Databáze: | OpenAIRE |
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