Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity
| Autor: | Richard A Lewis, Jason Micklefield, Laura Nunns, Colin P. Smith, Majid Al Nakeeb, Anna-Winona Struck, Matthew Q. Styles, Jenny Thirlway |
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| Rok vydání: | 2012 |
| Předmět: |
Glutamine
Streptomyces coelicolor Peptides Cyclic Catalysis Substrate Specificity Lipopeptides Protein structure Nonribosomal peptide Amino Acid Sequence Peptide Synthases Peptide sequence Adenylylation chemistry.chemical_classification biology Chemistry Active site General Chemistry General Medicine Anti-Bacterial Agents Protein Structure Tertiary Amino acid Enzyme Biochemistry Mutagenesis Site-Directed biology.protein Calcium |
| Zdroj: | Angewandte Chemie. 124:7293-7296 |
| ISSN: | 0044-8249 |
| DOI: | 10.1002/ange.201202043 |
| Popis: | Calcium-dependent antibiotics (CDA) are cyclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes. Active site modification of the 3-methyl glutamate activating adenylation (A) domain of the CDA NRPS enables the incorporation of synthetic 3-methyl glutamine into CDA. This provides the first example of how A-domains can be engineered to introduce synthetic "non-natural" amino acids into nonribosomal peptides. |
| Databáze: | OpenAIRE |
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