Expression of an idiotype (Id-460) during in vivo anti-dinitrophenyl antibody responses. I. Mapping of genes for Id-460 expression to the variable region of immunoglobulin heavy-chain locus and to the variable region of immunoglobulin kappa-light-chain locus
| Autor: | Charles A. Janeway, R W Rosenstein, P D Gottlieb, E A Dzierzak |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Idiotype
Genes MHC Class II Immunology Immunoglobulin Variable Region chemical and pharmacologic phenomena Locus (genetics) Biology Immunoglobulin light chain Major Histocompatibility Complex Immunoglobulin kappa-Chains Mice Immunoglobulin Idiotypes Animals Immunology and Allergy Gene Nitrobenzenes Regulator gene Genetics Structural gene Chromosome Mapping Articles Molecular biology Dinitrobenzenes Myeloma Proteins Antibody Formation Immunoglobulin heavy chain Immunoglobulin Light Chains Binding Sites Antibody Immunoglobulin Heavy Chains Antibody Diversity |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.152.3.720 |
| Popis: | The genetic contro of the expression of an idiotype (Id-460) associated with the 2,4-dinitrophenyl (DNP)-binding BALB/c myeloma protein MOPC 460 was studied using congenic strains of mice. It was shown that the expression of high levels of Id-460 during secondary in vivo anti-DNP-ovalbumin responses was determined by genes governing immunoglobulin heavy-chain variable and kappa-light chain variable regions (V kappa). Appropriate alleles at both loci were required for the expression of Id-460. Genes in the major histocompatability complex and the X-linked immune deficiency gene found in strain CBA/N did not greatly affect Id-460 expression. The V kappa gene controlling Id-460 expression can be differentiated from Lyt-3, and it is the first instance in which expression of an idiotype subdivides the V kappa genes associated with the Lyt-3a allele. Although it is likely that the V kappa gene(s) involved are structural, the involvememt of a regulatory gene linked to the structural gene can not be excluded. |
| Databáze: | OpenAIRE |
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