Fungal dermatitis, glossitis and disseminated visceral mycosis caused by different Metarhizium granulomatis genotypes in veiled chameleons ( Chamaeleo calyptratus ) and first isolation in healthy lizards
| Autor: | Michael Pees, Juliane Schneider, Linus Klasen, Volker Schmidt, Jens Hübel |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metarhizium Pogona Pathology medicine.medical_specialty Antifungal Agents Glossitis 040301 veterinary sciences 030106 microbiology Chamaeleo Microbiology 0403 veterinary science 03 medical and health sciences Drug Resistance Fungal Genotype medicine Animals Dermatomycoses Ribosomal DNA Phylogeny Mycosis General Veterinary biology Lizards 04 agricultural and veterinary sciences General Medicine Ribosomal RNA medicine.disease biology.organism_classification Viscera |
| Zdroj: | Veterinary Microbiology. 207:74-82 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2017.06.005 |
| Popis: | Metarhizium (M.) granulomatis (formerly Chamaeleomyces granulomatis) invariably causes fatal fungal glossitis and systemic mycosis in veiled chameleons (Chamaeleo calyptratus). Isolation of M. granulomatis in other lizards thus far has not been described. The aim of this study therefore was to obtain information on the presence of M. granulomatis in reptiles kept as pets, and to examine whether there was an association between specific genotypes and clinical/pathological outcomes. Besides 18S ribosomal (r) DNA (SSU) and internal transcribed spacer1-5.8S (ITS1-5.8S) rDNA, a fragment of the large subunit of the 28S rDNA (LSU), including the domains 1 (D1) and D2, were sequenced for identification of the fungus and phylogenetic analysis. Metarhizium granulomatis was isolated from 23 veiled chameleons, two panther chameleons (Furcifer pardalis) and one central bearded dragon (Pogona vitticeps). Only the veiled chameleons revealed corresponding pathological findings in the form of glossal hemorrhage, granulomatous glossitis, pharyngitis, dermatitis and/or visceral mycosis. The infection site correlated to survival times of infected veiled chameleons. Combined long-term treatment with terbinafine and nystatin based on susceptibility testing may be helpful for prevention of disease and visceral spreading of the fungus, but elimination of the fungal pathogen or successful treatment of diseased veiled chameleons have not been achieved yet. Sequencing of the ribosomal genes yielded five different genotypes, with genotype A being strongly correlated with dermatitis, and remaining genotypes with pharyngitis and glossitis. However, disseminated visceral mycosis developed irrespective of the genotypes. |
| Databáze: | OpenAIRE |
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