X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue
| Autor: | Alexander Hipp, Christoph Rau, Christian David, Andrew C. Cook, Christian Grünzweig, Joan Vila-Comamala, Daniel Dilg, Marie-Christine Zdora, Georg Schulz, Irene Zanette, Pierre Thibault, Anna Khimchenko |
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| Přispěvatelé: | Zdora, Marie-Christine, Vila-Comamala, Joan, Schulz, Georg, Khimchenko, Anna, Hipp, Alexander, Cook, Andrew C., Dilg, Daniel, David, Christian, Grünzweig, Christian, Rau, Christoph, Thibault, Pierre, Zanette, Irene |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Image quality Medical and biological imaging Tomography X-ray imaging X-ray interferometry X-ray microscopy 02 engineering and technology Grating Article 03 medical and health sciences Biological specimen Optics Optical coherence tomography Microscopy medicine medicine.diagnostic_test business.industry ocis:(170.6960) Tomography ocis:(110.7440) X-ray imaging 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Interferometry 030104 developmental biology Beamline ocis:(180.7460) X-ray microscopy ocis:(170.3880) Medical and biological imaging ocis:(340.7450) X-ray interferometry 0210 nano-technology business Biotechnology |
| Zdroj: | Zdora, M, Vila-comamala, J, Schulz, G, Khimchenko, A, Hipp, A, Cook, A C, Dilg, D, David, C, Grünzweig, C, Rau, C, Thibault, P & Zanette, I 2017, ' X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue ', Biomedical Optics Express, vol. 8, no. 2, pp. 1257 . https://doi.org/10.1364/BOE.8.001257 Biomedical Optics Express |
| DOI: | 10.1364/BOE.8.001257 |
| Popis: | The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM. |
| Databáze: | OpenAIRE |
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