Routine Use of a Protease Zymogen-Based Colorimetric Assay for the Detection of Beta-Glucan and its Role in Clinical Practice
| Autor: | Marco Conte, Silvana Sanna, Esther Manso, Claudio Farina, Paolo Fazii, Elisabetta Blasi, Silvana Perin, Stefano Andreoni, Dimitrios Panellis, Pietro Pini, Gianluigi Lombardi |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male beta-Glucans medicine.drug_class medicine.medical_treatment Immunology Antibiotics Aspergillosis Tazobactam Mannans Galactomannan chemistry.chemical_compound Antigen Zymogen Humans Immunology and Allergy Medicine skin and connective tissue diseases Aged Pharmacology Enzyme Precursors Aspergillus Beta-glucan Diagnosis Invasive aspergillosis Protease biology business.industry Galactose Middle Aged bacterial infections and mycoses medicine.disease biology.organism_classification Molecular biology carbohydrates (lipids) chemistry Colorimetry Female Proteoglycans business Peptide Hydrolases medicine.drug |
| Zdroj: | Scopus-Elsevier ResearcherID |
| ISSN: | 2058-7384 |
| DOI: | 10.1177/039463201402700424 |
| Popis: | The detection of Aspergillus antigen (galactomannan) is considered a reliable marker for the diagnosis of invasive aspergillosis (IA), yet the sensibility and specificity of the assays commonly employed in routine are not optimal. The aim of the present study was to investigate whether the detection of another panfungal antigen, the (1,3)-b-D-glucan could have an auxiliary role in the identification of patients with IA. The study was carried out on 63 sera belonging to patients who had been screened for galactomannan, according to the clinical suspect of IA. Our data show that the positive galactomannan results were not confirmed by positive (1,3)-b-D-glucan results in patients receiving therapy with beta-lactam antibiotics associated with tazobactam, whereas in all the other cases, with the exception of four, the results of the (1,3)-b-D-glucan test were confirmatory of the galactomannan results. |
| Databáze: | OpenAIRE |
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