Purification and isolation of corticosteroidogenic cells from head kidney of rainbow trout (Oncorhynchus mykiss) for testing cell-specific effects of a pesticide
| Autor: | Alice Hontela, Vincent S. Leblond, John P. Chang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Fish Proteins
endocrine system medicine.medical_specialty Cell type 3-Hydroxysteroid Dehydrogenases Hydrocortisone Cell Survival Physiology Health Toxicology and Mutagenesis Cell Separation Biology Kidney Toxicology Biochemistry Adrenocorticotropic Hormone Adrenal Cortex Hormones Internal medicine Centrifugation Density Gradient medicine Animals Centrifugation Lymphocytes Pesticides Cell Shape Head Kidney Dose-Response Relationship Drug Staining and Labeling urogenital system Differential staining Povidone Reproducibility of Results Kidney metabolism Cell Biology General Medicine Silicon Dioxide Molecular biology In vitro Endocrinology Oncorhynchus mykiss Rainbow trout Percoll Endosulfan |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 147:52-60 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2007.07.008 |
| Popis: | The teleost head kidneys contain corticosteroidogenic cells, chromaffin cells, lymphoid cells, and melanomacrophages. We have developed and validated a method using a Percoll density gradient and differential staining for 3beta-hydroxysteroid dehydrogenase (3beta-HSD) to prepare fractions enriched with specific head kidney cell types. The proportion of steroidogenic cells to other cells in the head kidney was 1:8000 in rainbow trout, Oncorhynchus mykiss. To test the hypothesis that steroidogenic cells are more vulnerable to a pesticide than other cell types in the head kidney, head kidney cells were separated by a Percoll gradient and the steroidogenic cell-enriched fractions and lymphoid cell-enriched fractions were exposed to the pesticide endosulfan in vitro, and their functional integrity and viability were assessed. The effective concentration of the pesticide (EC50, concentration that inhibits 50% of the secretory response to ACTH) in the mixed head kidney cell preparation was similar to the EC50 in the fraction enriched with steroidogenic cells, but differences in viability were detected. The Percoll method for isolation of different cell types from the head kidney facilitated a study of cell-specific effects of a pesticide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect