Differential cytokine modulation of the genes LAMA3, LAMB3, and LAMC2, encoding the constitutive polypeptides, α3, β3, and γ2, of human laminin 5 in epidermal keratinocytes
| Autor: | Alain Mauviel, Karsten Korang, Angela M. Christiano, Jouni Uitto |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Keratinocytes
TGF-β TGF alpha Biophysics Biology Biochemistry Mice Transforming Growth Factor beta Structural Biology Laminin Complementary DNA Gene expression Genetics medicine Animals Humans Molecular Biology Gene Cells Cultured Mice Inbred BALB C integumentary system Laminin 5 Cell Biology Molecular biology HaCaT medicine.anatomical_structure Epidermal Cells Gene Expression Regulation LAMC2 Gene TNF-α biology.protein Epidermis Peptides Keratinocyte Cell Adhesion Molecules |
| Zdroj: | FEBS Letters. (3):556-558 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(95)00740-Z |
| Popis: | Laminin 5, an anchoring filament protein previously known as nicein/kalinin/epiligrin, consists of three polypeptide chains, alpha 3, beta 3, and gamma 2, encoded by the genes LAMA3, LAMB3, and LAMC2, respectively. The expression of laminin 5 was detected by Northern hybridization with specific cDNA probes in various epidermal keratinocyte cultures, whereas no expression of any of the three genes could be detected in foreskin fibroblast cultures. Transforming growth factor-beta (TGF-beta) enhanced LAMA3, LAMB3, and LAMC2 gene expression in human epidermal keratinocytes, as well as in HaCaT and Balb/K cells in culture, although the extent of enhancement was greater for LAMA3 and LAMC2 genes than for LAMB3. Interestingly, tumor necrosis factor-alpha, (TNF-alpha) alone did not alter the expression of LAMB3 and LAMC2 genes in human epidermal keratinocytes, whereas it inhibited the expression of LAMA3. These results suggest that the expression of the three genes encoding the laminin 5 subunits is not coordinately regulated by the cytokines tested. |
| Databáze: | OpenAIRE |
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