Limited Genetic Variability Among American Isolates of Grapevine virus E from Vitis spp
| Autor: | Jeremy R. Thompson, Adib Rowhani, Fevziye Celebi-Toprak, M. Al Rwahnih, Marc Fuchs, J. Vargas-Asencio, Keith L. Perry |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetic diversity biology Grapevine virus E food and beverages Plant Science biology.organism_classification 01 natural sciences Virology law.invention 03 medical and health sciences 030104 developmental biology Intergenic region law Plant virus Genetic variation Genetic variability Agronomy and Crop Science Gene Polymerase chain reaction 010606 plant biology & botany |
| Zdroj: | Plant Disease. 100:159-163 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-05-15-0556-re |
| Popis: | A survey for the presence of Grapevine virus E (GVE, genus Vitivirus, family Betaflexiviridae) in vineyards in New York and California was conducted using macroarray hybridization or reverse-transcription polymerase chain reaction (RT-PCR) assays. In New York, GVE was detected in 10 of 46 vines of Vitis labrusca, one V. riparia, and one Vitis hybrid. All GVE-infected New York vines were coinfected with Grapevine leafroll-associated virus-3. In California, GVE was detected in 8 of 417 vines of V. vinifera. All GVE-infected California vines were also coinfected by one of the leafroll-associated viruses and other vitiviruses. In order to assess the genetic diversity among GVE isolates, a viral cDNA was amplified by RT-PCR, and a 675-nucleotide region that included the 3' terminus of the coat protein gene, a short intergenic region, and the 5' terminus of the putative nucleic acid binding protein gene was sequenced. All 20 GVE isolates sequenced in this study were very closely related, with >98% nucleotide identity to the SA94 isolate from South Africa. These findings confirm the presence of GVE in major grape-growing regions of the United States and indicate a very low level of genetic diversity. © 2016 The American Phytopathological Society. |
| Databáze: | OpenAIRE |
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