Structure determination of channel and transport proteins by high-resolution microscopy techniques
| Autor: | Dimitrios Fotiadis, Daniel Harder, Jean-Marc Jeckelmann, Marcel Meury, Zöhre Ucurum, Rajendra Boggavarapu |
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| Rok vydání: | 2011 |
| Předmět: |
0303 health sciences
Electron crystallography Chemistry 030302 biochemistry & molecular biology Clinical Biochemistry Membrane Proteins Nanotechnology Microscopy Atomic Force Biochemistry Transport protein Characterization (materials science) 03 medical and health sciences Protein Transport Scintillation proximity assay Membrane protein Transmission electron microscopy Microscopy Biophysics Lipid bilayer Molecular Biology 030304 developmental biology |
| Zdroj: | Meury, Marcel; Harder, Daniel; Ucurum, Zöhre; Boggavarapu, Rajendra; Jeckelmann, Jean-Marc; Fotiadis, Dimitrios (2011). Structure determination of channel and transport proteins by high-resolution microscopy techniques. Biological chemistry, 392(1-2), pp. 143-50. Berlin: Walter de Gruyter 10.1515/BC.2011.004 Biological chemistry |
| ISSN: | 1437-4315 |
| DOI: | 10.1515/BC.2011.004 |
| Popis: | High-resolution microscopy techniques provide a plethora of information on biological structures from the cellular level down to the molecular level. In this review, we present the unique capabilities of transmission electron and atomic force microscopy to assess the structure, oligomeric state, function and dynamics of channel and transport proteins in their native environment, the lipid bilayer. Most importantly, membrane proteins can be visualized in the frozen-hydrated state and in buffer solution by cryo-transmission electron and atomic force microscopy, respectively. We also illustrate the potential of the scintillation proximity assay to study substrate binding of detergent-solubilized transporters prior to crystallization and structural characterization. |
| Databáze: | OpenAIRE |
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