Detection of two DCCD-binding components in the envelope membrane of H. halobium

Autor:	Naoyuki Murakami, Tetsuya Konishi
Jazyk:	angličtina
Předmět:	Chromatography Chemistry Biophysics Cell Biology H. halobium envelope membrane DCCD-binding protein Biochemistry High-performance liquid chromatography H+ transport Membrane Structural Biology Genetics [14C]DCCD Molecular Biology Envelope (waves)
Zdroj:	FEBS Letters. (2):283-286
ISSN:	0014-5793
DOI:	10.1016/0014-5793(84)80334-8
Popis:	From labeling studies using [14C]DCCD, the presence of two distinct DCCD-binding sites was proved in halobacterial membrane, whose binding constants are 0.03 and 0.08 nmol DCCD/mg envelope protein, respectively. HPLC and SDS—PAGE analysis revealed that the radioactivity bound to these sites migrated with apparent molecular masses of 45 and 10.2 kDa protein components, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb32afebf9d1a45710e41a9463ae7869 Zobrazit plný text záznamu