BtuB-Dependent Infection of the T5-like Yersinia Phage ϕR2-01
| Autor: | Jin Woo Jun, Lotta Happonen, Mikael Skurnik, Maria Pajunen |
|---|---|
| Přispěvatelé: | Glycoscience Group, Biosciences, HUMI - Human Microbiome Research, Department of Bacteriology and Immunology, Medicum, Helsinki One Health (HOH), Research Programs Unit, Mikael Skurnik / Principal Investigator, HUSLAB |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
medicine.medical_treatment PROTEIN LIPOPOLYSACCHARIDE Yersinia Siphoviridae Bacteriophage phage ϕR2-01 bacteriophage COMPLETE GENOME SEQUENCE Bacteriophages Yersinia enterocolitica phage 4)R2-01 Bacteriophage T5 Genetics 0303 health sciences biology Escherichia phage T5 HOST RECEPTOR QR1-502 3. Good health Infectious Diseases Lytic cycle ENTEROCOLITICA ESCHERICHIA-COLI Bacterial Outer Membrane Proteins Phage therapy proteome Mutagenesis (molecular biology technique) STATISTICAL-MODEL Genome Viral Microbiology Article 03 medical and health sciences Bacterial Proteins Virology Escherichia medicine BtuB genome BACTERIOPHAGE-T5 STRUCTURE 030304 developmental biology 030306 microbiology Membrane Transport Proteins MASS-SPECTROMETRY biology.organism_classification VIRULENCE 3111 Biomedicine |
| Zdroj: | Viruses Volume 13 Issue 11 Viruses, Vol 13, Iss 2171, p 2171 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13112171 |
| Popis: | Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_phi R2-01 (in short, phi R2-01) that infects strains of several Yersinia enterocolitica serotypes. The phi R2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The phi R2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical phi R2-01 proteins. Label-free mass-spectrometry-based proteomics confirmed the expression of 90 of the phi R2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and phi R2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of phi R2-01 as a food biocontrol or phage therapy agent. |
| Databáze: | OpenAIRE |
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