RNA landscape of the emerging cancer-associated microbe Fusobacterium nucleatum
| Autor: | Falk Ponath, Caroline Tawk, Jörg Vogel, Yan Zhu, Franziska Faber, Lars Barquist |
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| Přispěvatelé: | HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Small RNA biology Fusobacterium nucleatum Immunology RNA Repressor Porins Fusobacteria Cell Biology Computational biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Bacterial genetics RNA Bacterial stomatognathic diseases Bacterial Proteins Neoplasms Gene expression Genetics Fusobacterium Infections Humans Gene |
| Zdroj: | Nature microbiology England |
| Popis: | Fusobacterium nucleatum, long known as a constituent of the oral microflora, has recently garnered renewed attention for its association with several different human cancers. The growing interest in this emerging cancer-associated bacterium contrasts with a paucity of knowledge about its basic gene expression features and physiological responses. As fusobacteria lack all established small RNA-associated proteins, post-transcriptional networks in these bacteria are also unknown. In the present study, using differential RNA-sequencing, we generate high-resolution global RNA maps for five clinically relevant fusobacterial strains-F. nucleatum subspecies nucleatum, animalis, polymorphum and vincentii, as well as F. periodonticum-for early, mid-exponential growth and early stationary phase. These data are made available in an online browser, and we use these to uncover fundamental aspects of fusobacterial gene expression architecture and a suite of non-coding RNAs. Developing a vector for functional analysis of fusobacterial genes, we discover a conserved fusobacterial oxygen-induced small RNA, FoxI, which serves as a post-transcriptional repressor of the major outer membrane porin FomA. Our findings provide a crucial step towards delineating the regulatory networks enabling F. nucleatum adaptation to different environments, which may elucidate how these bacteria colonize different compartments of the human body. |
| Databáze: | OpenAIRE |
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