Establishment and characterization of a bovine mammary epithelial cell line with unique properties
| Autor: | M van Duffelen, Ioannis Politis, B. Zavizion, Warren I. Schaeffer |
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| Rok vydání: | 1996 |
| Předmět: |
Cellular differentiation
Antigens Polyomavirus Transforming Blotting Western Genetic Vectors Biology Mammary Glands Animal Epidermal growth factor Cytotoxic T cell Animals Microscopy Phase-Contrast Antigen-presenting cell Growth Substances Cell Line Transformed A549 cell Confluency Cell Differentiation Epithelial Cells Cell Biology General Medicine Cell Transformation Viral Molecular biology Culture Media Microscopy Electron Microscopy Fluorescence Cell culture Cattle Stem cell Cell Division Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 32(3) |
| ISSN: | 1071-2690 |
| Popis: | Clonal cell lines (BME-UV) were established from primary epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T antigen. The BME-UV cells have undergone more than 300 population doublings and produce intranuclear large T antigen. At low confluency, growing islands of cells are apparent exhibiting the characteristic cobblestone morphology of epithelial cells. The BME-UV cells expressed functional markers such as microvilli and desmosomes and biochemical markers of mammary epithelial cells such as a repertoire of cytokeratins. The BME-UV cells are capable of synthesizing low levels of alpha-lactalbumin and alpha s1-casein (50 ng/ml of medium/24 h). One of the cell lines, BME-UV1 showed enhanced proliferation in the presence of epidermal growth factor (EGF) and insulinlike growth factor I (IGF-I). The BME-UV1 cell line is the only known bovine mammary epithelial cell line responsive to EGF. The BME-UV cells grown on collagen at low confluency are capable of developing very long projections that most likely allow for communication between cells at a distance from each other. The BME-UV cells may become a valid model system to examine bovine mammary epithelial proliferation and differentiation and cell-to-cell communication. |
| Databáze: | OpenAIRE |
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