Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
Autor: | Ivana Jarić, Jasmina Živanović, Branko Filipović, Sanja Stankovic, Vladimir Ajdžanović, Verica Milošević, Marko Miler |
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Rok vydání: | 2021 |
Předmět: |
Male
medicine.medical_specialty MAP Kinase Signaling System Parathyroid hormone Genistein 030209 endocrinology & metabolism Andropause Phosphates 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Homeostasis Rats Wistar Protein kinase A Protein kinase B Klotho 030304 developmental biology Mitogen-Activated Protein Kinase Kinases 0303 health sciences Kinase General Medicine Rats Disease Models Animal Endocrinology chemistry Calcium Anatomy Signal transduction Orchiectomy Developmental Biology |
Zdroj: | Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft. 239 |
ISSN: | 1618-0402 |
Popis: | Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis. |
Databáze: | OpenAIRE |
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