Identification of phosphatidylserylglutamate: a novel minor lipid in Escherichia coli
| Autor: | Rebecca L. Rose, Reza Kordestani, Teresa A. Garrett, Christian R. H. Raetz, Travis D. Richardson, Jennifer D. Son |
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| Rok vydání: | 2009 |
| Předmět: |
Cyclopropanes
Spectrometry Mass Electrospray Ionization Protein mass spectrometry Electrospray ionization electrospray ionization Analytical chemistry QD415-436 Phosphatidylserines medicine.disease_cause Mass spectrometry Biochemistry Dissociation (chemistry) Sample preparation in mass spectrometry Endocrinology Fragmentation (mass spectrometry) Escherichia coli medicine Phospholipids mass spectrometry Chromatography Escherichia coli K12 Ion exchange Chemistry Fatty Acids lipid structure determination Dipeptides Cell Biology Lipids Research Article |
| Zdroj: | Journal of Lipid Research, Vol 50, Iss 8, Pp 1589-1599 (2009) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.m800549-jlr200 |
| Popis: | Advances in mass spectrometry have facilitated the identification of novel lipid structures. In this work, we fractionated the lipids of Escherichia coli B and analyzed the fractions using negative-ion electrospray ionization mass spectrometry to reveal unknown lipid structures. Analysis of a fraction eluting with high salt from DEAE cellulose revealed a series of ions not corresponding to any of the known lipids of E. coli. The ions, with m/z 861.5, 875.5, 887.5, 889.5, and 915.5, were analyzed using collision-induced dissociation mass spectrometry (MS/MS) and yielded related fragmentation patterns consistent with a novel diacylated glycerophospholipid. Product ions arising by neutral loss of 216 mass units were observed with all of the unknowns. A corresponding negative product ion was also observed at m/z 215.0. Additional ions at m/z 197.0, 171.0, 146.0, and 128.0 were used to propose the novel structure phosphatidylserylglutamate (PSE). The hypothesized structure was confirmed by comparison with the MS/MS spectrum of a synthetic standard. Normal phase liquid chromatography-mass spectrometry analysis further showed that the endogenous PSE and synthetic PSE eluted with the same retention times. PSE was also observed in the equivalent anion exchange fractions of total lipids extracted from the wild-type E. coli K-12 strain MG1655. |
| Databáze: | OpenAIRE |
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