Determination of Unsulfonated Aromatic Amines in FD&C Yellow No. 5 and FD&C Yellow No. 6 by Liquid Chromatography–Triple Quadrupole Mass Spectrometry
| Autor: | Samuel R. White, Nebebech Belai |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
Electrospray Analyte Chromatography United States Food and Drug Administration Chemistry Extraction (chemistry) Food Coloring Agents Quechers United States Quantitative determination Benzidine Analytical Chemistry chemistry.chemical_compound Aniline Tandem Mass Spectrometry Triple quadrupole mass spectrometry Environmental Chemistry Amines Agronomy and Crop Science Chromatography High Pressure Liquid Food Analysis Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 102:580-589 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.18-0165 |
| Popis: | Background: This paper describes a simple and sensitive ultra-HPLC–triple quadrupole MS (LC-MS/MS) method for the determination of six unsulfonated aromatic amines in the color additives FD&C Yellow No. 5 (Y5) and FD&C Yellow No. 6 (Y6). The six amines determined by thismethod are aniline (ANL), benzidine (BNZ), 4-aminobiphenyl (4ABP), 4-aminoazobenzene (4AAB), 2-aminobiphenyl (2ABP), and 4-aminobenzonitrile (4ABN). Objective: This method is intended foruse in batch certification of the color additives by the U.S. Food and Drug Administration (FDA) to ensure that each lot meets published specifications for coloring foods, drugs, and cosmetics. Methods: A modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) procedure is used for extraction of the amines. Quantitative determination was performed in electrospray positive ionization and multiple-reaction monitoring modes. Results: Validation of the method demonstrated overall recovery of 101–115% and precision of 1.74–9.78% for all analytes. Excellent regression coefficients were obtained, with values >0.999. Conclusions: The validated method was successfully used for the analyses of 30 Y5 and Y6 samples and provided results that are consistent with results from the current method used by FDA, with greater sensitivity and low matrix effects. Highlights: The validation results demonstrate that the new LC-MS/MS method is applicable for use in routine batch certification. |
| Databáze: | OpenAIRE |
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