Effects of surface microtopography of titanium disks on cell proliferation and differentiation of osteoblast-like cells isolated from rat calvariae
| Autor: | Tetsuya Kikui, Yasushi Hibino, Hiroshi Kadokura, Hiroshi Kawazu, H. Shigeta, Perry R Klokkevold, Henry H. Takei, Hiroshi Nakajima, Satoshi Yokose |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Surface (mathematics) Materials science Surface Properties chemistry.chemical_element Biocompatible Materials In Vitro Techniques Osseointegration 03 medical and health sciences 0302 clinical medicine Calcification Physiologic Acid Etching Dental medicine Animals General Dentistry Cells Cultured Cell Proliferation Titanium Osteoblasts Cell growth Skull Osteoblast Cell Differentiation 030206 dentistry Smooth surface Rats 030104 developmental biology medicine.anatomical_structure chemistry Rough surface Calcium content Ceramics and Composites Biophysics Microscopy Electron Scanning Calcium |
| Zdroj: | Dental materials journal. 37(2) |
| ISSN: | 1881-1361 |
| Popis: | The surface topography of implant fixture is an important factor affecting the osseointegration. We herein demonstrated the effects of surface microtopography of titanium disks on proliferation and differentiation of osteoblast-like cells isolated from rat calvariae. Titanium disks with machine surface (MS), rough surface (R1) and rough surface combined with small cavities (R2) were used in an in vitro culture system. Rough surfaces (R1 and R2 disks) induced stronger osteoblast proliferation and differentiation (BGP and sclerostin mRNA expressions and calcium content) than the smooth surface (MS disk). Furthermore, surface microtopography of R2 disk, which was rough with small cavities, more strongly induced cell proliferation and mineralized bone matrix production than R1 disk. Our results suggest that surface microtopography influences osteoblast proliferation and differentiation. R2 disk, which is rough with small cavities, may be used in implant fixtures to increase osseointegration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|