Genotoxicity, acute and subchronic toxicity evaluation of savory food ingredients
| Autor: | Shahrzad Tafazoli, Nigel Baldwin, Phoukham Phothirath, Anne Petersen, Myriam Coulet, Johanna Lang, Anne Constable, Trung D. Vo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Male medicine.medical_specialty DMBA Gene mutation Toxicology medicine.disease_cause Kidney 01 natural sciences 03 medical and health sciences Ingredient 010608 biotechnology Internal medicine medicine Toxicity Tests Acute Animals Food science No-Observed-Adverse-Effect Level Hematology Chemistry Mutagenicity Tests Satureja Toxicity Tests Subchronic Food Ingredients Heart General Medicine Glutamic acid Rats 030104 developmental biology Biochemistry Liver Glycine Fermentation Female Genotoxicity DNA Damage Mutagens |
| Zdroj: | Regulatory toxicology and pharmacology : RTP. 87 |
| ISSN: | 1096-0295 |
| Popis: | The potential toxicity of two savory food ingredients produced by fermentation of enzymatically hydrolyzed corn starch (Savory Base 100 and Savory Base 200) was evaluated individually in a bacterial reverse mutation assay, an in vitro mammalian cell gene mutation assay, an acute oral study and as a mixture in a 90-day dietary study. In the bacterial reverse mutation and in vitro mammalian cell gene mutation assays, neither ingredient was mutagenic at concentrations up to 5000 μg/plate and 5000 μg/mL, respectively in the presence and absence of metabolic activation. In the acute study, the no-observed-adverse-effect level (NOAEL) for each Savory Base 100 and Savory Base 200 in male and female rats was 2000 mg/kg body weight. In the 90-day study, the hematology and clinical chemistry findings and histopathological changes noted in the liver, heart and kidneys were deemed to be of no toxicological significance, as the mean values were within the historical control range, were not dose-dependent, occurred at a similar frequency in control groups, or only occurred in the control group. Considering these findings, the NOAEL for Savory Base 100 and Savory Base 200 was 2333 and 1167 mg/kg body weight, respectively, the highest dose tested in each case. |
| Databáze: | OpenAIRE |
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