Transmission ofYersinia pestisfrom an Infectious Biofilm in the Flea Vector
| Autor: | B. Joseph Hinnebusch, Scott D. Kobayashi, Clayton O. Jarrett, Eszter Deak, Adeline R. Whitney, Karen E. Isherwood, Frank R. DeLeo, Elizabeth R. Fischer, Petra C. F. Oyston |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Flea Neutrophils Yersinia pestis Microbiology Evolution Molecular Extracellular matrix chemistry.chemical_compound Bacterial Proteins Immunity Disease Transmission Infectious Animals Immunology and Allergy Immunity Cellular Plague biology Biofilm biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae Virology Insect Vectors Gastrointestinal Tract Infectious Diseases chemistry Biofilms Siphonaptera Female Bacteria Hemin |
| Zdroj: | The Journal of Infectious Diseases. 190:783-792 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/422695 |
| Popis: | Transmission of plague by fleas depends on infection of the proventricular valve in the insect's foregut by a dense aggregate of Yersinia pestis. Proventricular infection requires the Y. pestis hemin storage (hms) genes; here, we show that the hms genes are also required to produce an extracellular matrix and a biofilm in vitro, supporting the hypothesis that a transmissible infection in the flea depends on the development of a biofilm on the hydrophobic, acellular surface of spines that line the interior of the proventriculus. The development of biofilm and proventricular infection did not depend on the 3 Y. pestis quorum-sensing systems. The extracellular matrix enveloping the Y. pestis biofilm in the flea appeared to incorporate components from the flea's blood meal, and bacteria released from the biofilm were more resistant to human polymorphonuclear leukocytes than were in vitro-grown Y. pestis. Enabling arthropod-borne transmission represents a novel function of a bacterial biofilm. |
| Databáze: | OpenAIRE |
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