Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR
| Autor: | Roberto M. La Ragione, Daniela Florio, Renato Giulio Zanoni, Adele Williams, Andy E. Durham, Guido Cordoni |
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| Přispěvatelé: | Cordoni, Guido, Williams, Adele, Durham, Andy, Florio, Daniela, Zanoni, Renato Giulio, La Ragione, Roberto M. |
| Rok vydání: | 2015 |
| Předmět: |
Time Factors
Streptococcus equi animal diseases Persistently infected Biology Classic and Real Time (RT) direct multiplex PCR Polymerase Chain Reaction Sensitivity and Specificity Streptococcus equi subsp. equi Microbiology law.invention Streptococcus equi subspecies equi Streptococcus equi subsp. zooepidemicu law Streptococcal Infections Animals Multiplex Horses Polymerase chain reaction Strangles General Veterinary Medicine (all) Strangles diagnosi Diagnostic test bacterial infections and mycoses Virology Equine/horse Streptococcus equi subsp. zooepidemicus Veterinary (all) Horse Diseases |
| Zdroj: | Research in Veterinary Science. 102:162-166 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2015.08.008 |
| Popis: | Strangles is one of the most common equine infectious diseases with serious health, welfare and socio-economic impact. However, the detection of Streptococcus equi subspecies equi can be challenging and persistently infected carriers are common. Furthermore, the use of classical microbiology can result in an underestimation of the prevalence of the disease. The difficulties associated with the slow diagnosis of Strangles can result in rapid spread of the disease. Therefore, rapid and economical diagnostic tests are urgently required. Here, two multiplex assays, were developed and validated for the detection of S. equi and S. equi subspecies zooepidemicus, the most common differential diagnosis. Using 59 S. equi and 59 S. zooepidemicus strains collected from various geographical areas, the PCR tests demonstrated a sensitivity of 95% and a specificity of 98%. Furthermore, the assay can be performed directly from clinical swabs. Thus, the assays designed here provide a rapid, reliable and economical solution for the diagnosis of Strangles. |
| Databáze: | OpenAIRE |
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