A Forward Genetic Screen for Suppressors of Somatic P Granules inCaenorhabditis elegans
| Autor: | Hannah E. Lust, Karolina M. Andralojc, Anne C. Campbell, Monique E. Theriault, Michael Senter-Zapata, Dustin L. Updike, Ashley L. Kelly |
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| Rok vydání: | 2015 |
| Předmět: |
synMuv B
Somatic cell Gene Expression Biology germline medicine.disease_cause retinoblastoma Germline Chromatin remodeling Animals Genetically Modified P granules 03 medical and health sciences 0302 clinical medicine Genes Reporter RNA interference Genetics medicine Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Alleles Genetics (clinical) 030304 developmental biology 0303 health sciences Mutation Mutant Screen Report Chromosome Mapping RNA-Binding Proteins biology.organism_classification Chromatin Repressor Proteins Germ Cells 030220 oncology & carcinogenesis C. elegans RNA Interference Genetic screen |
| Zdroj: | G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation. |
| Databáze: | OpenAIRE |
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