Orchestrating serine resolvases
| Autor: | Sherwin P. Montaño, Phoebe A. Rice, Kent W. Mouw, Sally J. Rowland, W. Marshall Stark, Martin R. Boocock |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Protein Conformation Biology Models Biological Biochemistry Article Recombinases Serine Enzyme activator Protein structure Bacterial Proteins Recombinase Animals Humans Protein–DNA interaction Recombination Genetic Synaptosome DNA Enzyme Activation DNA Nucleotidyltransferases Biophysics Nucleic Acid Conformation DNA supercoil Energy source |
| Zdroj: | Biochemical Society Transactions. 38:384-387 |
| ISSN: | 1470-8752 0300-5127 |
| DOI: | 10.1042/bst0380384 |
| Popis: | A remarkable feature of the serine resolvases is their regulation: the wild-type enzymes will catalyse intra- but not inter-molecular recombination, can sense the relative orientation of their sites and can exchange strands directionally, despite the fact that there is no net release of chemical bond energy. The key to this regulation is that they are only active within a large intertwined complex called the ‘synaptosome’. Because substrate topology greatly facilitates (or, in other cases, inhibits) formation of the synaptosome, it acts as a ‘topological filter’. Within the defined topology of the synaptosome, strand exchange releases supercoiling tension, providing an energy source to bias the reaction direction. The regulatory portion of this complex contains additional copies of the recombinase and sometimes other DNA-bending proteins. We are using a combination of X-ray crystallography, biochemistry and genetics to model the full synaptic complex and to understand how the regulatory portion activates the crossover-site-bound recombinases. |
| Databáze: | OpenAIRE |
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