Ex vivo evaluation of the stability, safety and antibacterial efficacy of an extemporaneous povidone‐iodine preparation for ophthalmic applications
| Autor: | Ilva D. Rupenthal, Gamith L. Kumarasinghe, Hans Vellara, Darina Khun, Vijayalekshmi Sarojini, Stephen Lee, Gayan Heruka De Zoysa, Sachin S. Thakur |
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| Rok vydání: | 2019 |
| Předmět: |
Staphylococcus aureus
medicine.medical_specialty medicine.drug_class chemistry.chemical_element Microbial Sensitivity Tests Antibacterial efficacy Iodine medicine.disease_cause Cornea 03 medical and health sciences 0302 clinical medicine Drug Stability Antiseptic medicine Animals Povidone-Iodine business.industry Ocular Infections Dermatology Anti-Bacterial Agents Ophthalmology medicine.anatomical_structure chemistry Pseudomonas aeruginosa 030221 ophthalmology & optometry Cattle Ophthalmic Solutions Irritation business 030217 neurology & neurosurgery Ex vivo Optometry |
| Zdroj: | Clinical and Experimental Optometry. 102:583-589 |
| ISSN: | 1444-0938 0816-4622 |
| DOI: | 10.1111/cxo.12899 |
| Popis: | Povidone-iodine is used as a cost-effective broad-spectrum antiseptic in the prophylaxis and treatment of certain ocular infections. In this study, the stability, ophthalmic irritation potential and antibacterial efficacy of an extemporaneous povidone-iodine preparation was determined using established ex vivo and in vitro assays.Extemporaneous iodine was prepared by simple dilution in normal saline. Preparation stability was evaluated by monitoring concentration and pH. Ocular safety was determined using the bovine cornea opacity and permeability assay. Efficacy was assessed by determining the minimum inhibitory and minimum bactericidal concentration of the preparation on Staphylococcus aureus and Pseudomonas aeruginosa.Diluted povidone-iodine maintained its stability over the 28-day evaluation. The formulation caused mild ocular irritation at the lowest prepared concentration (0.5 per cent w/v), with irritation noticeably increased at higher concentrations. The preparation showed minimum bactericidal and inhibitory concentrations of 0.078 and 0.3 per cent w/v on S. aureus and P. aeruginosa, respectively.This study confirms the stability and broad-spectrum antibacterial efficacy of povidone-iodine, while addressing the ocular irritation potential of this chemical. |
| Databáze: | OpenAIRE |
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