Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose

Autor: Shaoqing Lei, Qing-Tao Meng, Rui Xue, Ling-Hua Tang, Zhongyuan Xia, Bo Zhao, Zi-ying Shen, Qian Sun, Rong Chen
Rok vydání: 2016
Předmět:
0301 basic medicine
DJ-1
Time Factors
Protein Deglycase DJ-1
medicine.disease_cause
Antioxidants
Kidney Tubules
Proximal
chemistry.chemical_compound
Malondialdehyde
oxidative stress
Articles
General Medicine
Cell Hypoxia
high glucose
Cystine
medicine.symptom
medicine.medical_specialty
Cell Survival
NF-E2-Related Factor 2
Blotting
Western
Biology
Transfection
Cell Line
Superoxide dismutase
03 medical and health sciences
Lactate dehydrogenase
Internal medicine
Genetics
medicine
Animals
Viability assay
Dose-Response Relationship
Drug
Renal ischemia
Superoxide Dismutase
NRK-52E cells
Epithelial Cells
hypoxia/reoxygenation injury
Hypoxia (medical)
Molecular biology
Rats
Oxygen
Glucose
030104 developmental biology
Endocrinology
chemistry
Apoptosis
biology.protein
Heme Oxygenase-1
Oxidative stress
Zdroj: International Journal of Molecular Medicine
ISSN: 1791-244X
1107-3756
DOI: 10.3892/ijmm.2016.2680
Popis: Patients with diabetes are more vulnerable to renal ischemia/reperfusion (I/R) injury, which is implicated in hyperglycemia-induced oxidative stress. We previously reported that the hyperglycemia-induced inhibition of DJ-1, a novel oncogene that exhibits potent antioxidant activity, is implicated in the severity of myocardial I/R injury. In the present study, we aimed to explore the role of DJ-1 in hypoxia/reoxygenation (H/R) injury in renal cells exposed to high glucose (HG). For this purpose, NRK-52E cells were exposed to HG (30 mM) for 48 h and then exposed to hypoxia for 4 h and reoxygenation for 2 h, which significantly decreased cell viability and superoxide dismutase (SOD) activity, and increased the malondialdehyde (MDA) content, accompanied by a decrease in DJ-1 protein expression. The overexpression of DJ-1 by transfection with a DJ-1 overexpression plasmid exerted protective effects against HG-induced H/R injury, as evidenced by increased CCK-8 levels and SOD activity, the decreased release of lactate dehydrogenase (LDH) and the decreased MDA content, and increased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) expression. Similar effects were observed following treatment with the antioxidant, N-acetylcysteine. These results suggest that the overexpression of DJ-1 reduces oxidative stress and attenuates H/R injury in NRK-52E cells exposed to HG.
Databáze: OpenAIRE
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