Growth Inhibition, Residual Contact and Translaminar Toxicity of Annona-based Bioinsecticides on Tomato Leafminer: Laboratory and Greenhouse Assessments

Autor: Leila Gimenes, Gabriel Luiz Padoan Gonçalves, Edson Luiz Lopes Baldin, Leandro do Prado Ribeiro, Elaine Ferrari de Brito, João B. Fernandes
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Universidade Federal de São Carlos (UFSCar), Agr Res & Rural Extens Co Santa Catarina
Rok vydání: 2019
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1439-0345
0367-4223
DOI: 10.1007/s10343-019-00493-3
Popis: Made available in DSpace on 2020-12-10T17:01:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-29 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study aimed to evaluate the bioactivity of ethanolic extracts from different parts of some Annona species (A. montana, A. muricata and A. sylvatica) against T. absoluta. In the initial screening, the ethanolic extracts from leaves and seeds of A. muricata promoted pronounced lethality and growth inhibition of T. absoluta larvae by means of residual contact and translaminar action. However, these extracts did not affect its biology, oviposition in choice and no-choice tests, as well as egg viability. Afterwards, the crude ethanolic extracts of leaves and seeds from A. muricata were submitted to liquid-liquid partitioning, and their respective fractions were evaluated against T. absoluta larvae. These procedures disclosed the hydroalcoholic fraction from A. muricata seeds and both the ethyl acetate and dichloromethane fractions from its leaves as the most active against T. absoluta larvae. Then, proton nuclear magnetic resonance (H-1 NMR) experiments were performed aiming to identify the main constituents present in these fractions. The chemical analyses of each NMR spectrum revealed that A. muricata bioactive fractions presented acetogenins as major compounds. Moreover, a formulated ethanolic extract from A. muricata seeds presented similar bioactivity against T. absoluta larvae in both laboratory and greenhouse bioassays when compared with two commercial botanical insecticides (Anosom(TM) 1 EC and Azaamax 1.2 EC). Therefore, the seeds from A. muricata, discarded during the process of its fruit pulp extraction, are a potential source of bioactive acetogenins to formulate botanical insecticides to control populations of T. absoluta in tomato crops, mainly in organic production systems. Sao Paulo State Univ, Sch Agr, Dept Crop Protect, Botucatu, SP, Brazil Univ Sao Paulo, ESALQ, Luiz de Queiroz Coll Agr, Dept Entomol & Acarol, Piracicaba, SP, Brazil Fed Univ Sao Carlos UFSCar, Dept Chem, Nat Prod Lab, Sao Carlos, SP, Brazil Agr Res & Rural Extens Co Santa Catarina, EPAGRI, CEPAF, Res Ctr Family Agr, Chapeco, SC, Brazil Sao Paulo State Univ, Sch Agr, Dept Crop Protect, Botucatu, SP, Brazil
Databáze: OpenAIRE
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