High-throughput genome-wide genotyping to optimize the use of natural genetic resources in the grassland species perennial ryegrass ( Lolium perenne L.)
| Autor: | José Luis Blanco-Pastor, Isabel Roldán-Ruiz, Philippe Barre, Fabien Surault, Thomas Ledauphin, Hilde Muylle, Matthew J. Hegarty, Jean Paul Sampoux, E. Willner, Thomas Keep, Tom Ruttink, Isabelle Litrico, Klaus J. Dehmer, Anna M. Roschanski |
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| Přispěvatelé: | Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères (P3F), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] (IPK-Gatersleben), Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Research Institute for Agricultural, Fisheries and Food (ILVO), Bourse de thèse cofinancée INRAE-Région Nouvelle Aquitaine |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences Genotype forage species Genome-wide association study Biology QH426-470 genebank association study 01 natural sciences Lolium perenne 03 medical and health sciences Germany Lolium Genetics GWAS Ecosystem Shared data resources Genetic variability Molecular Biology Genotyping Genetics (clinical) genomic prediction 030304 developmental biology 2. Zero hunger natural diversity 0303 health sciences Genetic diversity Ecotype Ecology business.industry Genetic Variation 15. Life on land biology.organism_classification Grassland Europe Plant Breeding GenPred Agriculture business 010606 plant biology & botany |
| Zdroj: | G3 G3, Genetics Society of America, 2020, 10 (9), pp.3347-3364. ⟨10.1534/g3.120.401491⟩ G3: Genes, Genomes, Genetics, Vol 10, Iss 9, Pp 3347-3364 (2020) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | The natural genetic diversity of agricultural species is an essential genetic resource for breeding programs aiming to improve their ecosystem and production services. A large natural ecotype diversity is usually available for most grassland species. This could be used to recombine natural climatic adaptations and agronomic value to create improved populations of grassland species adapted to future regional climates. However describing natural genetic resources can be long and costly. Molecular markers may provide useful information to help this task. This opportunity was investigated for Lolium perenne L., using a set of 385 accessions from the natural diversity of this species collected right across Europe and provided by genebanks of several countries. For each of these populations, genotyping provided the allele frequencies of 189,781 SNP markers. GWAS were implemented for over 30 agronomic and/or putatively adaptive traits recorded in three climatically contrasted locations (France, Belgium, Germany). Significant associations were detected for hundreds of markers despite a strong confounding effect of the genetic background; most of them pertained to phenology traits. It is likely that genetic variability in these traits has had an important contribution to environmental adaptation and ecotype differentiation. Genomic prediction models calibrated using natural diversity were found to be highly effective to describe natural populations for almost all traits as well as commercial synthetic populations for some important traits such as disease resistance, spring growth or phenological traits. These results will certainly be valuable information to help the use of natural genetic resources of other species. |
| Databáze: | OpenAIRE |
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