Alterations in gene expression in cultured human cells after acute exposure to uranium salt: Involvement of a mineralization regulator
| Autor: | Eric Quéméneur, Nicole Sage, Sylvie Ruat, Odette Prat, Gérard Steinmetz, Frédéric Bérenguer |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Blotting Western Gene Expression Biology Toxicology Kidney Gene expression medicine Humans Osteopontin Gene Lung Cells Cultured Oligonucleotide Array Sequence Analysis Minerals Microscopy Confocal Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Kidney metabolism Membrane Proteins General Medicine Molecular biology Uranium Compounds Phosphoric Monoester Hydrolases Blot Kinetics Proteinuria medicine.anatomical_structure Cell culture biology.protein RNA Spectrophotometry Ultraviolet DNA microarray |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 24(1) |
| ISSN: | 1879-3177 |
| Popis: | The risk of exposure of workers or populations to materials, such as uranium, of nuclear fuel process origins is a major concern worldwide. Our goal is to improve the knowledge of mechanisms ruling its chemical toxicity, and to search for proteins as potential indicator of effect. Such a marker of internal damage remains to be discovered in the case of uranium. This study, based on DNA microarrays, reports a comparative gene expression analysis following acute uranium exposure of several human cell lines taken from kidneys or lungs as representative targets. Among uranium altered genes, no common gene was found between cells originating from lungs and kidney. In contrast, a set of 24 altered genes was common to two kidney cell lines. Transcriptional levels of a subset of renal genes were assessed with qRT-PCR. Furthermore, we highlighted a gene (SPP1) coding for a secreted protein (osteopontin) linked to ectopic mineralization. Immunoblotting assays showed that uranyl ions affect the excretion of osteopontin in a time- and dose-dependent manner. We consider that osteopontin, described as associated with bone resorbtion and kidney mineral stones, is a worthwhile candidate to be tested in vivo as a potential indicator of uranyl mineralization effects. |
| Databáze: | OpenAIRE |
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